ACE及Arg16Gly基因与哮喘患儿临床预后及治疗反应的关联  

Association of ACE and Arg16Gly Genes with Clinical Prognosis and Treatment Response in Children with Asthma

作　　者：刘丁香[1] LIU Dingxiang(Suining Central Hospital,Sichuan Suining 629000,China)

机构地区：[1]四川省遂宁市中心医院儿科,四川遂宁629000

出　　处：《河北医学》2022年第7期1099-1103,共5页Hebei Medicine

基　　金：四川省科技计划项目,(编号:2019YFS0037)。

摘　　要：目的:分析ACE及Arg16Gly基因与哮喘患儿临床预后及治疗反应的关联。方法:筛选我院2020年1月至2021年1月收治的140例哮喘患儿,使用DNA测序及荧光定量聚合酶链式反应(PCR)技术对其口腔黏膜细胞哮喘相关基因进行检测。对比不同类型基因突变患儿临床预后特点及糖皮质激素治疗反应,分析儿童哮喘基因无创检测指导临床预后评估及合理用药的价值。结果:140例患儿ACE I/D基因型以DD为主,D基因与I基因的优势比为1.98;ADRB2 Arg16Gly基因型以AG为主,G基因与A基因的优势比为1.89。ACE I/D基因型为DD型患儿病情控制率、治疗总有效率低于Ⅱ型、ID型患儿。ADRB2 Arg16Gly基因型为GG型患儿病情控制率、治疗总有效率低于AA型、AG型患儿。结论:哮喘患儿哮喘基因相关位点单核苷酸多态性(SNP)表达存在明显差异且与患儿临床预后、药物治疗反应密切相关,ACE I/D基因型DD型、ADRB2 Arg16Gly基因型GG型患儿临床预后普遍较差且对糖皮质激素治疗反应有限。Objective:To analyze the relationship between ACE and Arg16Gly genes and clinical prognosis and treatment response of children with asthma.Methods:A total of 140 children with asthma admitted to our hospital from January 2020 to January 2021 were screened,and the genes related to asthma in oral mucosa cells were detected by DNA sequencing and fluorescent quantitative polymerase chain reaction(PCR).The clinical prognosis characteristics of children with different types of gene mutations and the response to glucocorticoid treatment were compared,and the value of non-invasive detection of asthma genes in children to guide clinical prognosis evaluation and rational drug use were analyzed.Results:The ACE I/D genotype of 140 children was DD,and the dominance ratio of D gene to I gene was 1.98.Adr2arg16Gly genotype is mainly AG,and the dominance ratio of G gene to A gene is 1.89.The disease control rate and total effective rate of ACE I/D genotype DD children are lower than those of type II and ID children.The disease control rate and total effective rate of children with ADR2ARG16Gly genotype GG are lower than those of children with AA and AG genotype.Conclusion:There are significant differences in the expression of SNP in asthma gene-related sites in children with asthma,and it is closely related to the clinical prognosis and drug treatment response of children.Children with ACE I/D genotype DD type and ADRB2 Arg16Gly genotype GG type generally have poor clinical prognosis and limited response to glucocorticoid treatment.

关 键 词：哮喘基因 基因检测 预后评估 合理用药 

分 类 号：R725.6[医药卫生—儿科]