右美托咪定调控circ_0072995对直肠癌细胞SW480增殖凋亡的研究  

Dexmedetomidine regulates the proliferation and apoptosis of rectal cancer cell SW480 by circ_0072995

作　　者：王冰 吕艳玲[1] WANG Bing;LV Yan-ling(Department of Anesthesiology,Baoji People's Hospital,Baoji 721000,Shaanxi,CHINA)

机构地区：[1]宝鸡市人民医院麻醉科,陕西宝鸡721000

出　　处：《海南医学》2022年第14期1769-1773,共5页Hainan Medical Journal

摘　　要：目的探讨右美托咪定对直肠癌细胞增殖、凋亡的影响及分子机制。方法收集2017年1月至2021年1月宝鸡市人民医院收治的88例直肠癌患者的癌组织及癌旁组织标本。采用实时荧光定量PCR(RT-qPCR)检测肠癌组织和细胞中circ_0072995的表达水平;分析circ_0072995表达与临床病理关系。SW480细胞分为con组、Dex 1μmol/L组、Dex 2μmol/L组、Dex 5μmol/L组、si-circ_0072995组、si-NC组、Dex+pcDNA-circ_0072995组、Dex+pcDNA组;MTT法检测细胞增殖抑制率;克隆形成实验检测克隆形成数;流式细胞术检测细胞凋亡率。结果直肠癌组织中circ_0072995表达水平为2.36±0.15,明显高于癌旁组织的1.00±0.02,差异有统计学意义(P<0.05);circ_0072995表达水平与TNM分期及淋巴结转移有关(P<0.05);不同浓度右美托咪定处理后SW480细胞增殖抑制率和凋亡率升高,克隆形成数减少,circ_0072995表达水平降低,呈浓度依赖性,差异均有统计学意义(P<0.05);沉默circ_0072995后,SW480细胞增殖抑制率和凋亡率升高,克隆形成数减少,差异均有统计学意义(P<0.05);与Dex+pcDNA组比较,Dex+pcDNA-circ_0072995组circ_0072995表达水平升高,细胞增殖抑制率和凋亡率降低,克隆形成数增加,差异均有统计学意义(P<0.05)。结论右美托咪定可通过下调circ_0072995抑制直肠癌细胞增殖,促进凋亡。Objective To explore the effect of dexmedetomidine on the proliferation and apoptosis of rectal cancer cells and its molecular mechanism.Methods The cancer tissues and paracancerous tissues of 88 patients with colorectal cancer from January 2017 to January 2021 in Baoji People's Hospital were collected.Real-time fluorescent quantitative PCR(RT-qPCR)was used to detect the expression level of circ_0072995 in colorectal cancer tissues and cells.The relationship between circ_0072995 expression and clinicopathology was analyzed.SW480 cells were divided into con group,Dex 1μmol/L group,Dex 2μmol/L group,Dex 5μmol/L group,si-circ_0072995 group,si-NC group,Dex+pcDNA-circ_0072995 group,Dex+pcDNA group.MTT method was used to detect cell proliferation inhibition rate.Clone formation test was used to detect the number of clones formed.Flow cytometry was used to detect the apoptosis rate.Results The expression level of circ_0072995 in rectal cancer tissues was higher than that in adjacent tissues:(2.36±0.15)vs(1.00±0.02),P<0.05.The expression level of circ_0072995 was correlated with TNM stage and lymph node metastasis(P<0.05).After treatment with different concentrations of dexmedetomidine,the proliferation inhibition rate and apoptosis rate of SW480 cells increased,the number of clones decreased,and the expression level of circ_0072995 was decreased,in a concentration-dependent manner,with statistically significant differences(P<0.05).After silencing circ_0072995,the proliferation inhibition rate and apoptosis rate of SW480 cells were increased,and the number of clones was decreased,with statistically significant differences(P<0.05).Compared with the Dex+pcDNA group,the expression level of circ_0072995 in the Dex+pcDNA-circ_0072995 group was increased,the cell proliferation inhibition rate and apoptosis rate were decreased,and the number of clones was increased,with statistically significant differences(P<0.05).Conclusion Dexmedetomidine can inhibit the proliferation of rectal cancer cells and promote apoptosis by down

关 键 词：直肠癌 右美托咪定 circ_0072995 SW480细胞 增殖 凋亡 

分 类 号：R735.37[医药卫生—肿瘤]