抗核抗体滴度及p53抗体对原发性肝癌的诊断价值  

Diagnostic value of antinuclear antibody titer and p53 antibody in primary hepatic carcinoma

作　　者：陈世豪[1] 林耀威 汤翠霞 CHEN Shi-hao;LIN Yao-wei;TANG Cui-xia(Department of Clinical Laboratory,Jiangmen Xinhui District People's Hospital,Jiangmen 529100,Guangdong,CHINA)

机构地区：[1]江门市新会区人民医院检验科,广东江门529100

出　　处：《海南医学》2022年第14期1793-1796,共4页Hainan Medical Journal

基　　金：广东省江门市科技计划项目(编号:2017A4050)。

摘　　要：目的探究抗核抗体(ANA)滴度及p53抗体对原发性肝癌(PHC)的诊断价值。方法选择2020年2月至2021年6月江门市新会区人民医院收治的50例PHC患者作为PHC组,选择同期50例自身免疫性肝炎(AIH)患者作为AIH组,另选择同期50例健康体检者作为对照组。比较三组受检者的ANA阳性率、p53抗体水平,比较PHC组、AIH组患者的ANA核型结果;采用Logistic回归分析法分析ANA、p53抗体与PHC诊断的相关性;绘制血清ANA、p53抗体以及联合检测诊断PHC受试者的工作特征曲线(ROC),计算并比较各自的曲线下面积(AUC)、敏感度、特异度和准确度。结果PHC组患者的ANA阳性率、p53抗体分别为62.00%、(1.21±0.28)kU/L,明显高于AIH组的14.00%、(0.98±0.25)kU/L,PHC组、AIH组的ANA阳性率、p53抗体水平明显高于对照组的0、(0.15±0.03)kU/L,差异均有统计学意义(P<0.05);PHC组患者的中斑点型、核仁型、着丝点型和胞浆型的比例分别为16.00%、14.00%、12.00%、16.00%,明显高于AIH组的2.00%、2.00%、0、2.00%,差异均有统计学意义(P<0.05);Logistic回归分析结果显示,ANA、p53抗体均与PHC的诊断密切相关(P<0.05);ANA、p53抗体以及联合检测诊断PHC的AUC分别为0.730、0.729、0.805,联合检测的敏感度、特异度、准确度分别为86.00%、92.00%、89.00%,明显高于ANA、p53抗体单项检测,差异均有统计学意义(P<0.05)。结论ANA、p53抗体是诊断PHC的血清标志物,两者联合检测能够显著提高PHC诊断的准确率,能够为PHC患者的早期治疗、病情和预后评估提供重要依据。Objective To study the diagnostic value of antinuclear antibody(ANA)titer and p53 antibody in primary hepatic carcinoma(PHC).Methods Fifty patients with PHC and 50 patients with autoimmune hepatitis(AIH)who were treated in Jiangmen Xinhui District People's Hospital from February 2020 to June 2021 were collected as PHC group and AIH group,respectively,and another 50 healthy persons in the same period were selected as the control group.The ANA positive rate and p53 antibody of the three groups were compared,and the ANA karyotype results of PHC group and AIH group were compared.Logistic regression was used to analyze the correlation between ANA,p53 antibody and the diagnosis of PHC.The receiver operating characteristic curves(ROC)of serum ANA,p53 antibody and combined detection in the diagnosis of PHC were drawn,and the area under the curve(AUC),sensitivity,specificity,and accuracy were calculated and compared.Results The positive rate of ANA and the level of p53 antibody in PHC group were 62.00%and(1.21±0.28)kU/L,respectively,significantly higher than 14.00%and(0.98±0.25)kU/L in AIH group;the ANA positive rate,the levels of p53 antibody in PHC group and AIH group were significantly higher than 0,(0.15±0.03)KU/L in the control group;the differences were statistically significant(P<0.05).The proportions of spotted type,nucleolar type,centromeric type,and cytoplasmic type in PHC group were 16.00%,14.00%,12.00%,and 16.00%,respectively,which were significantly higher than 2.00%,2.00%,0,and 2.00%in AIH group(P<0.05).Logistic regression analysis showed that ANA,p53 antibody were closely related to the diagnosis of PHC(P<0.05).The AUC of ANA,p53 antibody,and combined detection in the diagnosis of PHC were 0.730,0.729,0.805,respectively.The sensitivity,specificity,and accuracy of combined detection were 86.00%,92.00%,and 89.00%,respectively,which were significantly higher than those of ANA,p53 antibody alone(P<0.05).Conclusion ANA,p53 antibody are serum markers for the diagnosis of PHC.The combined detection can signific

关 键 词：抗核抗体滴度 P53抗体 原发性肝癌 自身免疫性肝炎 阳性率 相关性 

分 类 号：R735.7[医药卫生—肿瘤]