低能量微波照射对大鼠正畸牙移动及牙周组织改建的影响  

Effect of low-energy microwave irradiation on orthodontic tooth movement and periodontal tissue remodeling in rats

作　　者：刘维佳 唐镇[1] 张琦 姜莉萍[1] LIU Wei-jia;TANG Zhen;ZHANG Qi;JIANG Li-ping(Affiliated Stomatological Hospital of Nanchang University,the Key Laboratory of Oral Biomedicine of Jiangxi Province,Jiangxi Province Clinical Research Center for Oral Diseases.Nanchang 330006,Jiangxi Province,China)

机构地区：[1]南昌大学附属口腔医院,江西省口腔生物医学重点实验室,江西省口腔疾病临床医学研究中心,江西南昌330006

出　　处：《上海口腔医学》2022年第2期156-161,共6页Shanghai Journal of Stomatology

基　　金：江西省自然科学基金(20202BABL206068)。

摘　　要：目的:探讨低能量微波照射对大鼠正畸牙移动及牙周组织改建的影响。方法:将SD大鼠随机分为对照组和实验组。采用螺旋弹簧加力法,通过镍钛拉簧装置构建大鼠正畸模型。实验组每天1次,用微波治疗仪照射被移动的第一磨牙,每次30 min;对照组不施加任何干预措施。分别在造模当天、第7、14、21天处死大鼠,测量大鼠第一磨牙的移动距离。抗酒石酸酸性磷酸酶(TRAP)染色检测大鼠牙周组织中破骨细胞计数;免疫组织化学检测破骨细胞分化因子(ODF)的表达;实时荧光定量PCR(RT-PCR)检测白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)mRNA的表达。采用SPSS 20.0软件包对实验数据进行统计学分析。结果:第7、14、21天时,与对照组相比,实验组大鼠第一磨牙移动距离、牙周组织中破骨细胞计数、ODF表达显著升高(P<0.05),牙周组织中IL-6和TNF-α的mRNA表达显著下降(P<0.05)。结论:低能量微波照射能明显加快正畸牙移动速度,抑制炎性基因表达,促进牙周组织改建。PURPOSE:To investigate the effect of low-energy microwave irradiation on the movement of orthodontic teeth and periodontal tissue reconstruction in rats.METHODS:SD rats were randomly divided into control group and experimental group.Helical spring force method was used to construct a rat orthodontic model through a nickel-titanium tension spring device.Rats in the experimental group were irradiated with a microwave treatment apparatus once a day to move the first molars for 30 minutes,while rats in the control group were not given any intervention.The rats were sacrificed on the day of modeling,7 d,14 d,and 21 d thereafter.The movement distance of the rat’s first molars was measured.Tartrate-resistant acid phosphatase(TRAP)staining was used to detect osteoclast counts in rat periodontal tissues,immunohistochemistry was used to detect the expression of cell differentiation factor(osteoclast differentiation factor,ODF)in rat periodontal tissues;real-time fluorescence quantitative PCR(RT-PCR)was used to detect the mRNA expression of interleukin 6(IL-6)and tumor necrosis factor-α(TNF-α).SPSS 20.0 software package was used to analyze the experimental data.RESULTS:At 7,14,21 d,compared with the control group,the distance of the first molar movement,the count of osteoclasts in the periodontal tissue,and the expression of ODF in the experimental group were significantly increased(P<0.05),while the mRNA expression of IL-6 and TNF-αin periodontal tissues was significantly decreased(P<0.05).CONCLUSIONS:Low-energy microwave irradiation can significantly accelerate the movement of orthodontic teeth,inhibit the expression of inflammatory genes,and promote the reconstruction of periodontal tissue.

关 键 词：低能量微波照射 正畸牙移动 牙周组织改建 炎性基因 

分 类 号：R783.5[医药卫生—口腔医学]