波纹龙虾全长转录组测序分析  

Sequencing Analysis of Full-length Transcriptome of Panulirus homarus

作　　者：梁妃爽 梁华芳[1] 孙榕泽 徐思行 温崇庆[1] 王伟[1] LIANG Fei-shuang;LIANG Hua-fang;SUN Rong-ze(Fisheries College,Guangdong Ocean University,Zhanjiang,Guangdong 524088)

机构地区：[1]广东海洋大学水产学院,广东湛江524088

出　　处：《安徽农业科学》2022年第14期92-97,100,共7页Journal of Anhui Agricultural Sciences

摘　　要：[目的]获得波纹龙虾丰富的转录组信息和功能基因。[方法]利用太平洋生物平台单分子实时测序技术,对波纹龙虾鳃、肝胰腺、肌肉、性腺、眼柄等组织混合进行全长转录组测序,获得波纹龙虾全长转录文库。[结果]单分子产出的高质量测序reads总量为71.57 G,通过测序拼接和去冗余后共获得10425个Unigene,平均长度为4147 bp。使用GO、NR、Pfam、NT、KEGG、SwissProt、KOG等数据库对全长序列进行基因功能注释,其中9580条序列得到NR数据库注释,分别比对到358个物种,其中与钩虾(Hyalella azteca)基因相似的序列最多,为3865个;有7585条序列得到GO注释;KOG功能注释将7436条单基因簇分为26个功能组分,其中一般功能预测的最多,为1421条;KEGG注释结果发现,心肌细胞的肾上腺素能信号、病毒性心肌炎、心脏肌肉收缩中注释的基因较多,分别为289、254和236条。[结论]通过全长转录组测序数据分析及功能注释,为进一步了解波纹龙虾的生物学特性、基因功能、相关代谢途径和信号通路等提供理论基础,为后续研究提供参考。[Objective]To obtain the rich transcriptome information and functional genes of the Panulirus homarus.[Method]This study used the Single-Molecule Real-Time sequencing technology of the PacBio platform to perform full-length transcriptome sequencing on mixed tissues of the Panulirush omarus gills,pancreas,muscles,gonads,and eyestalks.A full-length transcript library of Panulirus homarus was obtained.[Result]The total number of high-quality sequencing reads produced by a single molecule was 71.57 G.A total of 10425 single gene clusters.and 3105 long-chain non-coding RNA sequences were obtained after sequencing and de-redundancy.Functional annotation of full-length sequences using NR,NT,SwissProt,Pfam,GO,KOG,KEGG,and other databases were done.9580 sequences of these were annotated in the NR database,compared to 358 species of which Hyalellaazteca had the most similar sequences at 3865.7585 sequences have been annotated by GO.KOG functional annotation divided 7436 single gene clusters into 26 functional components,of which the most general functional prediction was 1421.KEGG annotation results found that the ADRENERGIC signal in the heart muscle cells pathways,viral myocarditis pathway and cardiac muscle contraction pathway have more annotated genes,thus 289,254,and 236 respectively.[Conclusion]Through full-length transcriptome sequencing data analysis and functional annotation,a theoretical basis for further understanding of the biological characteristics,gene functions,related metabolic pathways,and signal pathways of the corrugated Panulirus homarus has been provided.

关 键 词：波纹龙虾(Panulirus homarus) 全长转录组 三代测序 功能注释 

分 类 号：S917.4[农业科学—水产科学]