LINC00963靶向miR-525-5p调控LPS诱导的牙周膜细胞损伤的机制研究  

The mechanizm of LINC00963 targeting miR-525-5p in the regulation of LPS-induced periodontal ligament cell damage

作　　者：喻茂文 柳建磊 汤洪波 陈建军[1] 莫邦竹 YU Maowen;LIU Jianlei;TANG Hongbo;CHEN Jianjun;MO Bangzhu(Department of Experimental Medicine,Jintang Hospital,West China Hospital Sichuan University,China,Chengdu 610400;Chengdu Tongchang Medical Laboratory)

机构地区：[1]四川大学华西医院金堂医院实验医学科,成都610400 [2]成都同昌医学检验所 [3]金堂县第一人民医院

出　　处：《实用口腔医学杂志》2022年第4期455-460,共6页Journal of Practical Stomatology

基　　金：中央引导地方科技发展项目(编号:2021ZYD0083);四川省科技厅科技计划项目(编号:2020YJ0387);西南医科大学校级应用基础重点项目(编号:2021ZKZD010)。

摘　　要：目的:探讨长链非编码RNA LINC00963是否通过靶向miR-525-5p调控LPS诱导的牙周膜细胞损伤。方法:分离培养牙周膜细胞,分别转染si-LINC00963、miR-525-5p和anti-miR-525-5p,用LPS处理形成炎性损伤细胞模型。采用RT-qPCR、ELISA、流式细胞术、Western blot检测牙周膜细胞中LINC00963、miR-525-5p表达、炎症因子TNF-α、IL-1β水平、细胞凋亡率。双荧光素酶报告实验分析LINC00963对miR-525-5p的靶向调控。结果:转染si-LINC00963或miR-525-5p过表达降低了LPS诱导的牙周膜细胞中TNF-α、IL-1β水平、凋亡率、Bax蛋白水平,提高了Bcl-2蛋白水平(P<0.05)。LINC00963靶向调控miR-525-5p的表达。下调miR-525-5p表达逆转了si-LINC00963表达对LPS诱导的牙周膜细胞损伤的作用。结论:干扰LINC00963通过靶向miR-525-5p,抑制LPS诱导的牙周膜细胞凋亡和炎症因子表达。Objective:To investigate the effects of LINC00963 on LPS-induced periodontal ligament cell damage by targeting miR-525-5p.Methods:Periodontal ligament cells were in vitro cultured,and transfected with si-LINC00963,miR-525-5p and anti-miR-525-5p respectively.The cells were treated with LPS for the establishement of inflammatory damage cell model.RT-qPCR,ELISA,flow cytometry,Western blotting were performed to detect the expression of LINC00963,miR-525-5p,TNF-α,IL-1βand apoptosis rate of the cells.The dual luciferase reporter experiment was used to analyze the targeted regulation of miR-525-5p by LINC00963.Results:Interfering with LINC00963 or miR-525-5p overexpression reduced the levels of TNF-α,IL-1β,apoptosis rate and Bax protein levels in periodontal ligament cells induced by LPS,and increased Bcl-2 protein levels in the cells(P<0.05).LINC00963 targeted and regulated the expression of miR-525-5p.Down-regulation of miR-525-5p reversed the effects of interfering LINC00963 expression on LPS-induced periodontal ligament cell damage.Conclusion:Interfering LINC00963 inhibits LPS-induced apoptosis and inflammatory factor expression in periodontal ligament cells by targeting miR-525-5p.

关 键 词：牙周膜细胞 LPS 凋亡 炎症因子 LINC00963 miR-525-5p 

分 类 号：R781.4[医药卫生—口腔医学]