Effects of Ethyl Acetate Extract of Phyllanthus reticulatus Leaves on Autophagy-related Proteins Beclin-1,ATG5 and LC3 in Nude Mice Xenograft Model of HCC  

作　　者：Zhipeng XU Chenyan LIANG Cuiliu PAN Yunli TANG Jianfang FENG Tong HE 

机构地区：[1]Guangxi University of Chinese Medicine, Nanning 530020, China [2]Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China [3]Wuxuan County Maternal and Child Health Care Hospital, Laibin 545900, China

出　　处：《Medicinal Plant》2022年第3期43-47,52,共6页药用植物：英文版

基　　金：Supported by the 2018 Basic Ability Improvement Project of Young and Middle-aged Teachers in Guangxi Universities (2018KY0300;xp018034);the Education Department of Guangxi Zhuang Autonomous Region and Guangxi University of Traditional Chinese Medicine (2019XK089);Guangxi Science and Technology Base and Talent Project (GuiKe AD20238058);the Key Laboratory of TCM Pharmacology of Guangxi University of Traditional Chinese Medicine

摘　　要：[Objectives]To explore the effects of ethyl acetate extract of Phyllanthus reticulatus leaves on autophagy-related proteins Beclin-1,ATG5 and LC3 by immunohistochemistry,and to preliminarily explore their effects on autophagy.[Methods]BEL-7404 Hepatocellular Carcinoma(HCC)nude mice model was established,and blank group(same volume of pure water),positive control group(20 mg/kg fluorouracil),high dose drug group(600 mg/kg),and medium dose drug group(300 mg/kg),and low dose drug group(150 mg/kg)were set up.After 2 weeks of intragastric administration,the nude mice were sacrificed,and the tumor tissues were taken out,processed by immunohistochemistry,and then made into paraffin sections.Photos were taken under an optical microscope(10×40),and evaluation and analysis were performed with the aid of the Image-Pro Plus 6.0 image analysis software.Differences were calculated using SPSS 20.0 software.The effects of drugs on autophagy-related proteins LC3,Beclin-1 and ATG5 were observed.[Results]Compared with the blank group,the medium and high dose groups of ethyl acetate extract of P.reticulatus leaves had the effect of promoting the increase of autophagy-related proteins LC3,Beclin-1 and ATG5(P<0.05).However,there was no significant difference between the low dose group of ethyl acetate extract of P.reticulatus leaves and the blank group(P>0.05).[Conclusions]The ethyl acetate extract of P.reticulatus leaves has a promoting effect on autophagy-related proteins LC3,Beclin-1,and ATG5.

关 键 词：Ethyl acetate extract of Phyllanthus reticulatus leaves AUTOPHAGY Hepatocellular Carcinoma(HCC) Nude mice 

分 类 号：R285.5[医药卫生—中药学]