生长激素释放激素受体拮抗剂对翼状胬肉上皮细胞生长的影响及机制  

Effect and mechanism of growth hormone-releasing hormone receptor antagonist on human pterygium epithelial cells

作　　者：何倍婷 林宏亮 王盛 覃泳杰 张洪洋 HE Beiting;LIN Hongliang;WANG Sheng;QIN Yongjie;ZHANG Hongyang(School of Medicine,South China University of Technology,Guangzhou 510006,Guangdong Province,China;Guangdong Provincial People’s Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510080,Guangdong Province,China)

机构地区：[1]华南理工大学医学院,广东省广州市510006 [2]广东省人民医院,广东省医学科学院,广东省广州市510080

出　　处：《眼科新进展》2022年第7期520-523,共4页Recent Advances in Ophthalmology

基　　金：广东省自然科学基金项目(编号:2020A1515010103);广州市基础研究计划基础与应用基础研究项目(编号:202102080345);国家自然科学基金项目(编号:82171036)。

摘　　要：目的探讨生长激素释放激素受体(GHRH-R)拮抗剂对人翼状胬肉上皮细胞生长的影响及机制。方法采用原发性翼状胬肉患者术中所取的翼状胬肉头部进行组织块培养,模拟翼状胬肉生长模型,根据翼状胬肉组织的透明度和血管化程度分为静止期翼状胬肉组(8例)和活动期翼状胬肉组(16例),并选取正常结膜上皮组织(6例)作为正常结膜组。此外,采用10μmol·L^(-1)GHRH-R拮抗剂MIA602作用于8例活动期翼状胬肉组的胬肉组织作为MIA602干预组。记录各组翼状胬肉上皮细胞的生长距离,并采用免疫印迹实验和免疫荧光染色检测各组GHRH-R、p-ERK1/2和Caspase-3的相对表达量。结果与正常结膜组和静止期翼状胬肉组相比,活动期翼状胬肉组翼状胬肉上皮细胞的生长速度明显加快,并且GHRH-R和p-ERK1/2表达均显著增高,Caspase-3表达明显降低(均为P<0.05)。与活动期翼状胬肉组相比,MIA-602干预组翼状胬肉上皮细胞生长速度明显变慢,GHRH-R和p-ERK1/2蛋白表达均显著降低,Caspase-3的表达显著升高(均为P<0.05)。结论阻断GHRH-R能够有效地抑制翼状胬肉的生长并促使翼状胬肉上皮细胞凋亡,其机制可能与降低细胞增殖能力和促进细胞凋亡有关。Objective To investigate the effect and mechanism of growth hormone-releasing hormone receptor(GHRH-R)antagonist on human pterygium epithelial cells(PECs).Methods The heads of pterygium taken from patients with primary pterygium were selected for explant culture to simulate the pterygium growth model.According to transparency and vascularization,pterygiums were divided into the quiescent group(n=8)and the active group(n=16).Besides,normal conjunctival epitheliums(n=6)were selected into the normal control group.Eight active pterygium samples were treated with 10μmol·L^(-1)GHRH-R antagonist MIA602 and included in the MIA602 intervention group.The growth distance of PECs in each group was recorded.The relative expression levels of GHRH-R,phosphorylated extracellular signal-regulated kinase 1/2(p-ERK1/2),and cysteine protease-3(Caspase-3)were detected by Western blot and immunofluorescence.Results Compared with the normal control group and the quiescent group,PECs grew faster in the active group,the expression levels of GHRH-R and p-ERK1/2 were remarkably increased,while the expression level of Caspase-3 was significantly decreased(all P<0.05).Compared with the active group,PECs grew slower in the MIA602 intervention group,the expression levels of GHRH-R and p-ERK1/2 were dramatically decreased,while the expression level of Caspase-3 was significantly increased(all P<0.05).Conclusion Blockade of GHRH-R can effectively suppress the growth of pterygiums and promote PECs apoptosis.The mechanism may be related to proliferation inhibition and apoptosis promotion.

关 键 词：原发性翼状胬肉 翼状胬肉上皮细胞 生长激素释放激素受体 细胞增殖 细胞凋亡 

分 类 号：R777.33[医药卫生—眼科]