马兜铃科药用植物叶绿体基因组比较分析  

Comparison of chloroplast genomes of medicinal plants in Aristolochiaceae

作　　者：赵容[1] 尹舒悦 姜诚淏 薛嘉宁 刘畅[1] 蔡欣航 邢艳萍[1] 康廷国[1] ZHAO Rong;YIN Shu-yue;JIANG Cheng-hao;XUE Jia-ning;LIU Chang;CAI Xin-hang;XING Yan-ping;KANG Ting-guo(Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;School of Mathematics and Statistics,Beijing Institute of Technology,Beijing 100081,China)

机构地区：[1]辽宁中医药大学,辽宁大连116600 [2]北京理工大学数学与统计学院,北京100081

出　　处：《中国中药杂志》2022年第11期2932-2937,共6页China Journal of Chinese Materia Medica

基　　金：辽宁省卫生健康委员会项目(2018004,2019004);中央本级重大增减支项目(2060302)。

摘　　要：该研究对汉城细辛Asarum sieboldii f.seoulense的叶绿体基因组进行测序分析并与GenBank下载的马兜铃科植物叶绿体基因组结构特征进行比较,旨在明确汉城细辛叶绿体基因组特征及马兜铃科药用植物叶绿体基因组的差异性。采用高通量测序技术对汉城细辛叶绿体基因组进行测序、组装,利用REPuter等软件对11种马兜铃科药用植物叶绿体基因组的一般特征,重复序列、IR边界以及进化关系进行分析。研究结果表明汉城细辛基因组大小为167 293 bp, LSC(large single-copy)为89 840 bp, SSC(small single-copy)为21 415 bp, IR(inverted repeat)为28 019 bp, GC量为37.9%,注释到133个基因,其中包括89个蛋白编码基因,36个tRNA基因和8个rRNA基因。11种马兜铃科植物的叶绿体基因组长度为159 308~167 293 bp,基因数为130~134个。其中5种药用植物叶绿体基因组中均发现了P、F、R、C 4种类型的长重复序列和具有SSR序列位点,其中汉城细辛具有6种类型的SSR序列,系统发育树中汉城细辛和辽细辛聚为1支。研究将为马兜铃科药用植物的分类鉴定及系统发育等相关研究提供基础。In this study, the chloroplast genome of Asarum sieboldii f. seoulense was sequenced, analyzed, and compared with chloroplast genomes of other medicinal plants in Aristolochiaceae downloaded from GenBank, aiming to clarify the characteristics of the chloroplast genome of A. sieboldii f. seoulense and the differences in chloroplast genome among medicinal plants of Aristolochiaceae. To be specific, the chloroplast genome of A. sieboldii f. seoulense was sequenced and assembled by high-throughput sequencing, and the general characteristics, repeats, inverted repeat(IR) boundary, and phylogenetic relationship of the chloroplast genomes of 11 medicinal species in Aristolochiaceae were analyzed with REPuter. The result showed that the genome of A. sieboldii f. seoulense was 167 293 bp, with large single-copy(LSC) region of 89 840 bp, small single-copy(SSC) region of 21 415 bp, IR region of 28 019 bp, and GC content of 37.9%. A total of 133 genes were annotated, including 89 protein-coding genes, 36 tRNA genes and 8 rRNA genes. The chloroplast genomes of the 11 medicinal species were 159 308-167 293 bp, with 130-134 genes annotated. Forward(F), reverse(R), complement(C), and palindromic(P) long repeats and simple sequence repeat(SSR) were found in the chloroplast genomes of five species. Among them, A. sieboldii f. seoulense had six types of SSR. In the phylogenetic tree, A. sieboldii f. seoulense and A. heterotropoides were in the same clade. The result is expected to lay a basis for the classification, identification, and phylogeny of medicinal plants in Aristolochiaceae.

关 键 词：马兜铃科 汉城细辛 叶绿体基因组 重复序列 

分 类 号：S567[农业科学—中草药栽培]