聚乙烯微塑料对花生幼苗镉吸收及生理特征的影响  

Effects of polyethylene microplastics on cadmium absorption and physiological characteristics of peanut seedling

作　　者：宗海英 刘君[1] 郭晓红 李萌[3] 黄小丽 王芳丽 宋宁宁 ZONG Haiying;LIU Jun;GUO Xiaohong;LI Meng;HUANG Xiaoli;WANG Fangli;SONG Ningning(School of Resources and Environment,Qingdao Agricultural University,Qingdao 266109,China;School of Resources and Environmental Engineering,Ludong University,Yantai 264025,China;Shandong Institute of Sericulture,Yantai 264001,China;Central Laboratory of Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学资源与环境学院,山东青岛266109 [2]鲁东大学资源与环境工程学院,山东烟台264025 [3]山东省蚕业研究所,山东烟台264001 [4]青岛农业大学中心实验室,山东青岛266109

出　　处：《农业环境科学学报》2022年第7期1400-1407,共8页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(31800421);山东省自然科学基金项目(ZR2018PC004);莱州市受污染耕地安全利用集中推进区建设项目(6602421140)。

摘　　要：为探讨地膜源微塑料和镉(Cd)复合污染对作物生理特性及吸收Cd的影响,以聚乙烯微塑料(PE)和Cd为试验材料,采用液配试验探究PE(50 mg·L^(-1)^(-1)和200 mg·L^(-1))和Cd(25μmol·L^(-1)和100μmol·L^(-1))单一及复合污染条件下花生幼苗丙二醛(MDA)含量、抗氧化酶活性、根系活力及Cd含量的变化。结果表明,PE单一处理时,50 mg·L^(-1)PE显著提高了花生生物量,而200 mg·L^(-1)PE显著提高了花生体内MDA含量,并抑制了花生根系活力,降低了花生生物量。PE和Cd共存时,与不添加PE相比,两种浓度(50mg·L^(-1)和200 mg·L^(-1))PE均显著诱导了花生体内MDA的产生,且不同程度提高了花生抗氧化酶活性,抑制了根系活力、显著降低了花生生物量和促进了花生对Cd的吸收,尤其是Cd浓度为100 mg·L^(-1)时,50 mg·L^(-1)和200 mg·L^(-1)PE使得花生根系Cd吸收提高幅度分别为60.9%和78.8%。双因素分析显示,PE和Cd交互对花生幼苗生理特征和Cd吸收有显著影响,说明PE和Cd复合污染对作物的毒性产生协同作用。To better understand the effects of microplastics,cadmium(Cd),and their combination on the physiological characteristics and Cd uptake of crops,polyethylene microplastics(PE)and Cd were used as experimental materials to measure the variations in malondialdehyde(MDA)content,antioxidant enzyme activities,root activity,and Cd uptake after peanut seedlings were cultured in single and binary solutions of PE(50 mg·L^(-1) and 200 mg·L^(-1))and Cd(25μmol·L^(-1) and 100μmol·L^(-1)).Results showed that peanuts biomass significantly increased under low PE concentration(50 mg·L^(-1));however,peanut biomass significantly decreased under high PE concentration(200 mg·L^(-1)),which significantly increased MDA content and inhibited the root activity in single PE system.Compared to PE at 0 mg·L^(-1),PE at 50 mg·L^(-1) and 200 mg·L^(-1) significantly induced MDA production,increased antioxidant enzyme activities,inhibited root activity,reduced biomass,and promoted Cd absorption in peanut seedlings,especially under 100μmol·L^(-1) Cd.In addition,50 mg·L^(-1) and 200 mg·L^(-1) PE increased the Cd concentration in the root by 60.9%and 78.8%,respectively in the binary systems.Moreover,double factor variance analysis showed that PE and Cd interaction had significant effects on the physiological characteristics and plant Cd uptake.These results suggest that there is a synergistic toxicity on crop under MPs and Cd combined pollution.

关 键 词：聚乙烯微塑料 花生 CD吸收 生理特征 

分 类 号：X173[环境科学与工程—环境科学] X505