脂肪酸结合蛋白4在卵巢癌中的表达及其对卵巢癌细胞上皮间充质转化的影响  

Expression of Fatty Acid Binding Protein 4 in Ovarian Cancer and Its Effect of Epithelial-Mesenchymal Transformation in Ovarian Cancer Cells

作　　者：莫佳娅 苏引利 段浩然 张嘉琳 王秋杰 吴晗[1] 谢娅[1] MO Jiaya;SU Yinli;DUAN Haoran;ZHANG Jialin;WANG Qiujie;WU Han;XIE Ya(Department of Gynecology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院妇科,河南郑州450052

出　　处：《河南医学研究》2022年第14期2501-2506,共6页Henan Medical Research

基　　金：河南省科技厅科技攻关项目(192102310070)。

摘　　要：目的探讨脂肪酸结合蛋白4(FABP4)在卵巢癌组织中的表达及其对卵巢癌Hey A8细胞上皮间充质转化(EMT)的影响。方法采用定量反转录聚合酶链反应(qRT-PCR)和免疫印迹法检测收集的10对卵巢癌组织及其相对应的转移癌组织的FABP4 mRNA和FABP4蛋白的表达,使用Kaplan-Meier Plotter在线数据库分析FABP4表达与卵巢癌患者生存预后的关系。采用Transwell实验检测人卵巢癌A2780、SKOV3和Hey A8细胞的侵袭能力。将FABP4抑制剂作用于侵袭能力强的Hey A8细胞,采用免疫印迹法检测细胞FABP4、N-cadherin、E-cadherin、Vimentin蛋白的表达水平,采用划痕实验、Transwell实验检测细胞迁移和侵袭能力。结果转移癌组织中FABP4 mRNA和FABP4蛋白表达水平高于卵巢癌组织(P<0.05),数据库资料显示FABP4 mRNA表达与卵巢癌患者的总体生存期和无疾病进展期相关(P<0.05);Hey A8细胞的侵袭能力强于SKOV3和A2780细胞(P<0.05);应用FABP4抑制剂的Hey A8细胞的迁移和侵袭能力降低(P<0.05);EMT相关表型N-cadherin、Vimentin蛋白表达下降,E-cadherin蛋白表达增加(P<0.05)。结论FABP4可促进卵巢癌转移和EMT,调控卵巢癌的进展,可作为治疗卵巢癌的潜在靶点。Objective To investigate the expression of fatty acid binding protein 4(FABP4)in ovarian cancer and its effect of epithelial-mesenchymal transformation(EMT)in ovarian cancer Hey A8 cells.Methods The expressions of FABP4 mRNA and FABP4 protein in 10 pairs of ovarian cancer tissues and their corresponding metastatic cancer tissues were detected by quantitative reverse transcriptase-mediated polymerase chain reaction(qRT-PCR)and Western blotting.The relationship between FABP4 expression and the survival prognosis of ovarian cancer patients was analyzed by the Kaplan-Meier Plotter online database.The invasion abilities of human ovarian cancer A2780,SKOV3 and Hey A8 cells were detected by Transwell assays.FABP4 inhibitor was applied to strong invasive Hey A8 cells,and the expression levels of FABP4,N-cadherin,E-cadherin and Vimentin proteins were detected by Western blotting,and cell migration and invasion were detected by scratch and Transwell assays.Results The FABP4 mRNA and FABP4 protein expression in metastatic cancer tissues were higher than those in ovarian cancer tissues(P<0.05).The database data showed that FABP4 mRNA expression was related to the overall survival and progression-free survival of ovarian cancer patients(P<0.05).The invasion ability of Hey A8 cells was stronger than that of SKOV3 and A2780 cells(P<0.05).The migration and invasion of Hey A8 cells treated with FABP4 inhibitors were decreased(P<0.05).The expression of N-cadherin and Vimentin protein decreased and the expression of E-cadherin protein increased in EMT related phenotypes(P<0.05).Conclusion FABP4 can promote ovarian cancer metastasis and EMT,and regulate the progression of ovarian cancer,which can be used as a potential target for ovarian cancer therapy.

关 键 词：卵巢癌 脂肪酸结合蛋白4 上皮间充质转化 迁移 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]