Recovery of fertility in quinestrol-treated or diethylstilbestroltreated mice:Implications for rodent management  

作　　者：Ming LIU Rongcan LUO Hao WANG Guangming CAO Yanling WANG 

机构地区：[1]State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing,China [2]University of the Chinese Academy of Sciences,Beijing,China

出　　处：《Integrative Zoology》2017年第3期250-259,共10页整合动物学（英文版）

基　　金：supported by the National Natural Science Foundation of China(31201715);the STS pro­gram of the Chinese Academy of Sciences(KFJ-EW-STS-068-3);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB11050000).

摘　　要：Fertility control is an alternative strategy to traditional culling for the management of rodent pests.Previous studies have demonstrated that quinestrol is a potential contraceptive for male rodents,but the recovery of fertility in quinestrol-treated rodents has not been evaluated.This study used C57BL/6J mice to evaluate the recovery rate of male fertility after the administration of quinestrol.Diethylstilbestrol(DES),a non-steroid estrogenic compound,was used for comparison.Different groups of mice were treated with 1 mg/kg quinestrol,1 mg/kg DES,or castor oil separately for 7 days.These mice were then killed on days 8,22 and 50 respectively.Our results indicated that the weight of epididymides and seminal vesicles decreased significantly on days 8 and 22 in quinestrol/DES-treated mice,with extensive histological changes in the seminiferous tubules.Sperm concentrations in the cauda epididymal fluid were significantly reduced on days 8 and 22 in both quinestrol and DES treatment groups and on day 50 for the DES,but not the quinestrol group.Further analysis revealed that DES-treated mice exhibited a higher proportion of abnormal sperm accumulation in the epididymis,indicating that the normal sperm transportation to the cauda epididymis was blocked.Our results indicate that the anti-fertility effects on male mice given quinestrol were of shorter duration than for those receiving DES at the dose of 1 mg/kg body weight.

关 键 词：DIETHYLSTILBESTROL male infertility QUINESTROL recovery of fertility rodent fertility control 

分 类 号：R73[医药卫生—肿瘤]