一种微管吸持细胞泊松比测定方法  

A Method for Determing Poisson’s Ratio of Cells Based on Micropipette Aspiration Technique

作　　者：李孝伟[1,2] 张文杰 张丹[2] 袁惠宇 刘娜 LI Xiaowei;ZHANG Wenjie;ZHANG Dan;YUAN Huiyu;LIU Na(School of Mechanics and Engineering Scienceand Automation,Shanghai University,Shanghai 200444,China;School of Mechatronic Engineering and Automation,Shanghai University,Shanghai 200444,China)

机构地区：[1]上海大学力学与工程科学学院,上海200444 [2]上海大学机电工程与自动化学院,上海200444

出　　处：《医用生物力学》2022年第3期448-453,共6页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(62073208)。

摘　　要：目的提出一种基于微管吸持技术的细胞泊松比测定方法。方法基于变形对称性假设,通过提取微管吸持下细胞发生的挤压变形特征,根据广义虎克定律,推导细胞泊松比与变形量之间的解析表达式,依据细胞膜表面标记点的位置变化,实现细胞泊松比的准确测定。结果对前列腺癌细胞中的LNCaP细胞进行泊松比测定,结果表明,LNCaP细胞的泊松比在0.44~0.46之间,平均值为0.45;同一个细胞特征点选取位置不同,对泊松比计算结果的影响在1.6%的误差范围内。结论本文方法简单易行,可提高细胞泊松比的测量精度,有助于临床利用细胞力学特性进行细胞检测和筛选。Objective To propose a method for determing cell Poisson’s ratio based on micropipette aspiration technique.Methods Based on the assumption of deformation symmetry,the analytical expression between Poisson’s ratio and the amount of deformation was derived by extracting the extrusion deformation characteristics of the cells under micropipette aspiration according to the generalized Hooke’s law.The accurate determination of Poisson’s ratio of cells was realized according to position changes of markers on the surface of cell membrane.Results The Poisson’s ratio of LNCaP cells in prostate cancer cells was measured.The result showed that the Poisson’s ratio of LNCaP cells was between 0.44 and 0.46,with an average value of 0.45.The influence of the location of the same cell feature points on calculation results of Poisson’s ratio was within the error range of 1.6%.Conclusions This method is simple and feasible,can improve the measurement accuracy of Poisson’s ratio of cells,and is helpful for cell detection and screening by using cell mechanical properties in clinic.

关 键 词：泊松比 微管吸持法 LNCAP细胞 

分 类 号：R318.01[医药卫生—生物医学工程]