Effects of in ovo delivered xylo-and mannan-oligosaccharides on growth performance,intestinal immunity,cecal short-chain fatty acids,and cecal microbiota of broilers  

作　　者：Amit Kumar Singh Utsav Prakash Tiwari Birendra Mishra Rajesh Jha 

机构地区：[1]Department of Human Nutrition,Food and Animal Sciences,University of Hawaii at Manoa,1955 East-West Rd,Honolulu,HI 96822,USA

出　　处：《Journal of Animal Science and Biotechnology》2022年第4期1099-1114,共16页畜牧与生物技术杂志（英文版）

基　　金：supported by the USDA National Institute for Food and Agriculture,Hatch-Multistate Fund;by the College of Tropical Agriculture and Human Resources,University of Hawaii at Manoa,Honolulu,HI,USA

摘　　要：Background:This study investigated a novel in ovo feeding strategy to determine the prebiotic effects of xylo-and mannan-oligosaccharides(XOS and MOS)differing in the degree of polymerization.A total of 192 fertilized eggs were divided into 6 treatment groups:i)normal saline control(NSC),ii)xylotriose(XOS3),iii)xylotetraose(XOS4),iv)mannotriose(MOS3),v)mannotetraose(MOS4),and vi)no injection control(NIC),each containing 4 replicate trays with 8 eggs per replicate.On d 17 of incubation,3 mg of oligosaccharides(except for controls)dissolved in 0.5 mL of 0.85%normal saline were injected into the amnion of Cobb 500 broilers eggs.After hatch,the chicks were raised for 28 d under standard husbandry practices and were fed a commercial broilers diet ad libitum,and samples were collected periodically.Results:The hatchability,growth performance,and relative weights of breast,drumstick,liver,and proventriculus were not different among the treatments(P>0.05).The XOS3 injection increased the total short-chain fatty acid production at d 28 compared with both control groups(P<0.05).The villus height to crypt depth ratio was significantly higher in the XOS4 group than both controls on the hatch day(P<0.01)but were not different among any treatments on d 7 and 28(P>0.05).On the hatch day,the expression level of the CD3 gene(a T cell marker)was increased by XOS3,while the IL-10 gene(a marker of anti-inflammatory cytokine)was reduced by MOS4(P<0.05)compared with both controls.Compared with both controls,XOS3 exhibited a trend of reduction for IL-10(P=0.074).No cytokines or lymphocyte markers were affected by the treatments on d 7(P>0.05),except XOS4 increased IL-4 compared with NSC(P<0.05).The broilers in the MOS4 group had higher operational taxonomic units(OTUs)and had more differentially abundant taxa,including order Lactobacillales and family Leuconostocaceae(P<0.05)than both controls on d 28.The predictive functional profiling indicated that the linoleic acid metabolism pathway was enriched in the cecal microbiota of the XOS3

关 键 词：BROILERS Gut health Immunity in ovo Mannanoligosaccharides MICROBIOTA OLIGOSACCHARIDES PREBIOTICS XYLOOLIGOSACCHARIDES 

分 类 号：S831.5[农业科学—畜牧学]