IFN-α2b对HEL细胞PD-1/PD-L1信号通路的作用及其机制研究  

The effect of interferon-α2b on PD-1/PD-L1 signaling pathway of HEL cells

作　　者：齐林 成志勇[2] 张丽军[1] 王梦 王宝艳[2] QI Lin;CHENG Zhiyong;ZHANG Lijun;WANG Meng;WANG Baoyan(Graduate School,Chengde Medical University,Chengde 067000,China;Department of Hematology,No.1 Hospital of Baoding,Baoding 071000,China)

机构地区：[1]承德医学院研究生院,河北承德067000 [2]保定市第一医院血液内科,河北保定071000

出　　处：《医学研究与教育》2022年第3期1-8,共8页Medical Research and Education

基　　金：河北省重点研发计划项目(223777105D)。

摘　　要：目的研究IFN-α2b对JAK2 V617F阳性人红白血病HEL细胞增殖、凋亡的影响及对程序性死亡受体-1(PD-1)与程序性死亡配体-1(PD-L1)的影响。方法不同浓度IFN-α2b作用于人红白血病HEL细胞系,CCK-8检测细胞活力,Caspase-3/7活性检测试剂盒检测Caspase-3/7活性。荧光定量PCR检测JAK2、PD-1及PD-L1 mRNA表达水平,流式细胞术检测不同浓度IFN-α2b作用HEL后PD-1、PD-L1蛋白表达。结果不同浓度IFN-α2b能够剂量及时间依赖性抑制HEL细胞增殖。IFN-α2b能够剂量及时间依赖性增加HEL细胞Caspase-3/7活性;IFN-α2b能够剂量依赖性降低JAK2 V617F突变量,同时能够抑制HEL细胞PD-1 mRNA及蛋白表达水平,轻度上调PD-L1表达。结论IFN-α2b可能通过抑制HEL细胞增殖,并参与调控细胞PD-1/PD-L1表达。Objective To study the effects of interferon-α2b(IFN-α2b)on proliferation and apoptosis and the influence with programmed death-1(PD-1)and programmed death ligand-1(PD-L1)of JAK2 V617F positive human erythroleukemia HEL cells.Methods Different concentrations of IFN-α2b were applied to human erythroleukemia HEL cell lines.CCK-8 was used to detect cell viability and caspase-3/7 activity was detected by caspase-3/7 activity assay kit.The expression levels of JAK2,PD-1 and PD-L1 mRNA were detected by fluorescence quantitative PCR.Flow cytometry was used to detect the effects of different concentrations of interferon-α2b on the expression of PD-1 and PD-L1 protein in HEL.Results Different concentrations of IFN-α2b could inhibit HEL cell proliferation in time and dose-dependent manner.IFN-α2b could increase caspase-3/7 activity of HEL cells in a dose and time-dependent manner;IFN-α2b could reduce JAK2 V617F mutation in a dose-dependentmanner,inhibit the expression of PD-1 mRNA and protein,and slightly increase the expression of PD-L1.Conclusion IFN-α2b may inhibit HEL proliferation and participate in the regulation of PD-1/PD-L1 expression.

关 键 词：干扰素 JAK2 V617F突变 HEL细胞 程序性死亡受体-1 程序性死亡配体-1 

分 类 号：R733.3[医药卫生—肿瘤]