PTGER4、RUNX1和PTEN在视网膜母细胞瘤中的表达及与组织病理学的关系  

Expression of PTGER4,RUNX1 and PTEN in retinoblastoma and their relationship with histopathology

作　　者：张旭[1] 刘曦[1] 杨丹[1] 欧舟 曾韬[1] ZHANG Xu;LIU Xi;YANG Dan;OU Zhou;ZENG Tao(Department of Ophthalmology,the Second People's Hospital of Yibin,Yibin,Sichuan,China,644000)

机构地区：[1]四川省宜宾市第二人民医院眼科,四川宜宾644000

出　　处：《分子诊断与治疗杂志》2022年第7期1153-1157,共5页Journal of Molecular Diagnostics and Therapy

基　　金：四川省卫生厅科研课题(20171513)。

摘　　要：目的 研究前列腺素E2受体4(PTGER4)、矮小相关转录因子(RUNX1)及第10同源染色体丢失性磷酸酶-张力蛋白基因(PTEN)在视网膜母细胞瘤(Rb)中的表达及与组织病理学的关系。方法 选取2017年5月至2020年6月四川省宜宾市第二人民医院收入行手术治疗的88例Rb患者的视网膜组织标本为研究组,另选取62例因外伤摘除眼球的正常视网膜组织为对照组。比较两组PTGER4、RUNX1、PTEN的表达,并分析三者与临床病理特征的关系。对研究组进行为期1年的随访,分析影响Rb患者预后的危险因素及PTGER4、RUNX1、PTEN三项指标对Rb患者的诊断价值。结果 研究组PTGER4、RUNX1阳性表达率高于对照组,PTEN阳性表达率低于对照组,差异均有统计学意义(P<0.05)。PTGER4、RUNX1阳性及PTEN阴性患者的视神经受损情况、肿瘤有无转移、是否化疗及病理分型与TGER4、RUNX1阴性及PTEN阳性表达患者比较,差异具有统计学意义(P<0.05)。随访结束,88例患者死亡15例,存活73例。视神经受损、细胞瘤转移、病理分型(未分化型)、PTGER4(阳性)、RUNX1(阳性)、PTEN(阴性)为影响Rb患者预后的独立危险因素(P<0.05)。PTGER4、RUNX1及PTEN三者联合曲线下面积为0.979,大于三者单独检测(P<0.05)。结论 PTGER4、RUNX1、PTEN在Rb患者中呈异常表达,检测三者可为患者病情、预后评估提供可靠资料。Objective To study the expressions of prostaglandin receptor 4(PTGER4),runt-related protein(RUNX1)and the 10th homologous chromosome loss phosphatase and tensin homolog(PTEN)in retinoblastoma(Rb)and their relationship with histopathology. Methods The retinal tissue specimens of88 patients with Rb who were admitted to the Second People’s Hospital of Yibin City,Sichuan Province for surgical treatment from May 2017 to June 2020 were selected as the research group,and 62 normal retinal tissues from which the eyeballs were removed due to trauma were selected as the control group. The expressions of PTGER4,RUNX1 and PTEN were compared between the two groups. The relationship between the three and clinicopathological characteristics,and the risk factors affecting the prognosis of patients with Rb(all patients were followed up for 1 year after discharge)were analyzed. Results The positive expression rates of PTGER4 and RUNX1 in the study group were higher than those in the control group,while the positive expression rates of PTEN were lower than those in the control group. The difference was statistically significant(P<0.05). At the end of follow-up,15 of the 88 patients died and 73 survived. The optic nerve damage,tumor metastasis,chemotherapy and pathological classification in patients with PTGER4,RUNX1 negative and PTEN negative were compared with those with TGER4,RUNX1 negative and PTEN positive expression,and the difference was statistically significant(P<0.05). Optic nerve damage,cell tumor metastasis,pathological classification(undifferentiated),RUNX1(positive),PTEN(negative)were independent risk factors affecting the prognosis of Rb patients(P<0.05). The area under the combined curve of PTGER4,RUNX1 and PTEN was0.979,which was greater than that of the three independent detection(P<0.05). Conclusion PTGER4,RUNX1,and PTEN are abnormally expressed in Rb patients. The detection of the three can provide reliable data for the evaluation of the patient’s condition and prognosis.

关 键 词：视网膜母细胞瘤 PTGER4 RUNX1 PTEN 组织病理学 

分 类 号：R739.7[医药卫生—肿瘤]