环肌酸抑制大鼠牙周炎周围牙槽骨吸收的实验研究  

Experimental study on inhibition of alveolar bone resorption around periodontitis in rats by cyclocreatine

作　　者：李慧萍[1] 刘秀明[1] 徐伟峰[1] 甄锦泽[1] 沈达[2] 张善勇[1] LI Hui-ping;LIU Xiu-ming;XU Wei-feng;ZHEN Jin-ze;SHEN Da;ZHANG Shan-yong(Department of Oral Surgery,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai 200011;Department of Oral and Maxillofacial Surgery,Suzhou Municipal Hospital,Suzhou 215000,Jiangsu Province,China)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔外科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海200011 [2]苏州市立医院本部口腔颌面外科,江苏苏州215000

出　　处：《中国口腔颌面外科杂志》2022年第2期111-116,共6页China Journal of Oral and Maxillofacial Surgery

基　　金：上海市卫生和计划生育委员会面上项目(201740168)。

摘　　要：目的:通过体外和体内实验探讨环肌酸对牙周炎造成的牙槽骨吸收的抑制作用。方法:体外实验通过细胞活力测定、碱性磷酸酶染色和茜素红染色、抗酒石酸酸性磷酸酶染色和实时反转录聚合酶链反应(RT-PCR)等检测,评价环肌酸对成骨细胞和破骨细胞增殖和分化的影响。动物实验将20只大鼠分为4组,A组为对照组,B组采用牙周结扎+生理盐水注射,C组采用牙周结扎+低浓度环肌酸注射,D组采用牙周结扎+高浓度环肌酸注射。除对照组大鼠外,所有大鼠均在双侧上颌第一磨牙上结扎4周,然后对实验组进行牙周局部注射环肌酸2个月。收集上颌骨标本进行显微计算机体层扫描(Micro-CT)。采用SPSS 17.0软件包对测量数据进行统计学分析。结果:体外实验表明,环肌酸抑制破骨细胞的增殖和分化,但不影响成骨细胞。体内实验表明,局部应用环肌酸可防止牙槽骨丢失和增加牙槽嵴高度。结论:环肌酸可抑制破骨细胞增殖和分化,具有抑制大鼠牙周炎中破骨细胞形成和减少牙槽骨丢失的作用。PURPOSE:To evaluate the inhibitory effect of cyclocreatine on alveolar bone resorption caused by periodon-titis both in vitro and in vivo.METHODS:In vitro,the effects of cyclocreatine on proliferation and differentiation of os-teoblasts and osteoclasts were evaluated by cell viability assay,alkaline phosphatase staining(ALP)and alizarin red staining,tartrate resistant acid phosphatase(TRAP)staining and real-time reverse transcription PCR(RT-PCR).Twenty rats were divided into four groups,group A was as control group,group B with periodontal ligation+normal saline injection,group C with periodontal ligation+low concentration injection,group D with periodontal ligation+high concentration injection.Except the control group,all rats were ligated on bilateral maxillary first molars for 4 weeks,and then treated with local injection of cyclocreatine for 2 months.The maxilla was collected for further Micro-CT evaluation.SPSS 16.0 software package was used for statistical analysis of the data.RESULTS:In vitro,cyclocreatine inhibited the proliferation and differentiation of osteoclasts,but did not affect osteoblasts.In vivo,local application of cyclocreatine can prevent local alveolar bone loss and increase alveolar ridge height.CONCLUSIONS:Cyclocreatine can inhibit proliferation and differentiation of osteoclasts,inhibit formation of osteoclasts and reduce alveolar bone loss in rat with periodontitis.

关 键 词：环肌酸 牙周炎 牙槽骨缺损 大鼠 

分 类 号：R782.1[医药卫生—口腔医学]