新疆南疆地区某规模牛场牛病毒性腹泻病毒检测及基因分型  

Molecular detection and genotyping of bovine viral diarrhea virus in a large cattle farm in southern Xinjiang

作　　者：鱼海玮 黄跃杰[2] 朱广艺 马纾薏 王旭 胡建军[1] YU Haiwei;HUANG Yuejie;ZHU Guangyi;MA Shuyi;WANG Xu;HU Jianjun(College of Animal Science,Tarim University,Alar 843300,China;Veterinary Station of the First Division,Xinjiang Production and Construction Group,Alar 843300,China)

机构地区：[1]塔里木大学动物科学学院,新噩阿拉尔843300 [2]新疆生产建设兵团第一师畜牧兽医工作站,新疆阿拉尔843300

出　　处：《畜牧与兽医》2022年第7期59-64,共6页Animal Husbandry & Veterinary Medicine

基　　金：新疆生产建设兵团科技局现代农业科技攻关与成果转化资助项目(2016AC012);新疆生产建设兵团第一师科技局资助项目(2016XS03);新疆生产建设兵团重点领域科技攻关计划(2019AB029)。

摘　　要：为掌握新疆南疆某规模牛场牛病毒性腹泻病毒(BVDV)感染的基因型,将该规模牛场所采集的血清样本经BVDV抗原ELISA检测阳性后作为PCR检测样本,针对BVDV基因组中5′-UTR、N^(pro)基因设计特异性引物,进行RT-PCR扩增,然后将目的基因进行测序、BLAST比对和序列分析,并构建系统发育树。结果显示,该规模牛场4份样本皆为BVDV-1c型。本研究结果为南疆规模牛场制定科学合理的牛病毒性腹泻防控措施以及选择相应疫苗提供了理论参考。In order to identify the genotype of BVDV in a large-scale cattle farm in the southern Xinjiang area, the positive serum samples being tested by BVDV antigen ELISA were detected by RT-PCR(reverse transcription PCR) accompanied with specific primers of the 5′-UTR gene and the N^(pro)gene. Sequencing, blast alignment, sequence analysis and phylogenetic tree construction were analyzed to identify the genotype of BVDV. The results showed that all of these positive serum samples were confirmed as the BVDV-1 c genotype. This study provided reference for prevention and control of BVD in large-scale cattle farms in southern Xinjiang.

关 键 词：牛病毒性腹泻病 5′-UTR基因 N 基因 系统进化树 

分 类 号：S852.65[农业科学—基础兽医学]