睡眠剥夺状态下脑功能显像的多模态研究  

Multimodal study on cerebral functional imaging under sleep deprivation

作　　者：轩昂 付畅 孙萌萌 王莉 徐俊玲 Xuan Ang;Fu Chang;Sun Mengmeng;Wang Li;Xu Junling(Department of Nuclear Medicine,People′s Hospital of Zhengzhou University,Henan Provincial People′s Hospital,People′s Hospital of Henan UniversityHenan Key Laboratory of Novel Molecular Probes and Clinical Translation in Nuclear Medicine,Zhengzhou 450003,China;Medicine Research and Translation Center,Academy of Medical Sciences of Henan Province,Zhengzhou 450003,China)

机构地区：[1]郑州大学人民医院、河南省人民医院、河南大学人民医院核医学科、河南省核医学新型分子探针与临床转化医学重点实验室,郑州450003 [2]河南省医学科学院医学研究和转化中心,郑州450003

出　　处：《中华核医学与分子影像杂志》2022年第7期391-396,共6页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：河南省医学科技攻关计划(201701026)。

摘　　要：目的探讨睡眠剥夺状态下健康志愿者脑葡萄糖代谢和血流灌注的改变以及二者之间的关系,揭示睡眠剥夺后脑功能损伤的具体脑区定位。方法前瞻性选取2019年1月至2019年12月间郑州大学人民医院的17名健康志愿者[男8名、女9名,年龄(22.5±1.7)岁],对志愿者进行2次磁共振三维(3D)动脉自旋标记(ASL)及^(18)F-FDG PET/CT显像,第1次在正常睡眠后2 h,第2次在睡眠剥夺24 h后。应用统计参数图(SPM)软件对3D-ASL图像和^(18)F-FDG PET/CT图像处理,分别得到睡眠剥夺前后脑代谢及灌注差异激活图,再获得代谢及灌注差异共同激活脑区图。将异常激活的脑区设为ROI,获得其脑血流量(CBF)值及SUV比(SUVR)值(以小脑为参考区),采用配对t检验及Pearson相关分析对数据进行处理。结果受试者睡眠剥夺后脑代谢与脑灌注均减低,且异常脑区相似,脑代谢减低的脑区明显多于灌注减低的脑区;睡眠剥夺后代谢及灌注共同减低的脑区集中于额叶、颞叶、顶叶等脑区;睡眠剥夺后受试者左侧背外侧额上回CBF值及SUVR值有相关性(r=0.58,P=0.014)。受试者睡眠剥夺后全脑平均CBF值[(46.32±7.39)ml·100 g^(-1)·min^(-1)]及SUVR值(1.46±0.04)均低于睡眠剥夺前全脑平均CBF值[(54.91±6.51)ml·100 g^(-1)·min^(-1)]及SUVR值(1.53±0.06),差异均有统计学意义(t值:-2.67、-3.72,P值:0.012、0.001)。结论初步揭示了睡眠剥夺后受试者脑功能损伤的具体脑区定位。对于睡眠剥夺脑功能研究,^(18)F-FDG PET/CT与3D-ASL具有一致性且前者更为敏感。左侧背外侧额上回可能是睡眠剥夺后脑功能损伤的核心脑区。Objective To reveal the specific region location of brain function injury after sleep deprivation by exploring cerebral glucose metabolism and blood perfusion changes and the correlation between them in healthy volunteers of sleep deprivation.Methods From January 2019 to December 2019,a total of 17 healthy volunteers(8 males,9 females;age(22.5±1.7)years)from People′s Hospital of Zhengzhou University were enrolled prospectively.All patients accepted MRI three-dimensional(3D)arterial spin labeling(ASL)and ^(18)F-FDG PET/CT scanning at 2 h after normal sleep and after sleep deprivation of 24 h.Statistical parametric mapping(SPM)software was used for image processing,and brain metabolism and perfusion differences activation graphs before and after sleep deprivation were obtained respectively.Then the common activated brain regions were obtained as ROI.The cerebral blood flow(CBF)and the SUV ratio(SUVR;the cerebellum was the reference area)were calculated.Pearson correlation analysis and paired t test were used for data analysis.Results The cerebral metabolism and perfusion of the subjects after sleep deprivation were reduced,and the abnormal brain areas were similar.Brain areas with reduced metabolism were more than those with reduced perfusion.The brain areas with reduced metabolism and perfusion after sleep deprivation were commonly in the frontal lobe,temporal lobe,parietal lobe,etc.The CBF and SUVR value of left dorsolateral frontal gyrus after sleep deprivation were correlated(r=0.58,P=0.014).The mean CBF value((46.32±7.39)ml·100 g^(-1)·min^(-1))and SUVR value(1.46±0.04)of whole brain after sleep deprivation were lower than those before sleep deprivation((54.91±6.51)ml·100 g^(-1)·min^(-1),1.53±0.06;t values:-2.67,-3.72,P values:0.012,0.001).Conclusions The specific region′s location of brain function injury after sleep deprivation is preliminarily revealed.^(18)F-FDG PET/CT was more sensitive than 3D-ASL for brain function research of sleep deprivation and left dorsolateral frontal gyrus may be a ke

关 键 词：睡眠剥夺 正电子发射断层显像术 体层摄影术 X线计算机 氟脱氧葡萄糖F18 磁共振成像 

分 类 号：R740[医药卫生—神经病学与精神病学]