卡瓦胡椒素B对人胰腺癌细胞增殖和侵袭能力的影响及机制  

Effect and mechanism of flavokawain B on proliferation and invasion of human pancreatic cancer cells

作　　者：李悦 崔冬冰[2] 郑迪杰 王秀红 LI Yue;CUI Dongbing;ZHENG Dijie;WANG Xiuhong(Department of Nursing,Guizhou Medical University,Guiyang 550004,Guizhou,China;Center for Tissue Engineering and Stem Cell Research,Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Hepatobiliary Surgery,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学护理学院,贵州贵阳550004 [2]贵州医科大学组织工程与干细胞实验中心,贵州贵阳550004 [3]贵州医科大学附属医院肝胆外科,贵州贵阳550004

出　　处：《贵州医科大学学报》2022年第7期766-772,共7页Journal of Guizhou Medical University

基　　金：全国医学专业学位研究生教育指导委员会(中国学位与研究生教育学会医学专业工作委员会)项目(YX2019-03-02)。

摘　　要：目的探讨卡瓦胡椒素B(FKB)对人胰腺癌细胞增殖和侵袭能力的影响及机制。方法取对数生长期的人胰腺癌细胞株PANC-1和SW1990,分别设置对照组[0.1%二甲基亚砜(DMSO)]和5、10、20、40μmol/L FKB组,采用细胞增殖实验(CCK-8)和平板克隆形成实验检测各组胰腺癌细胞PANC-1和SW1990的增殖能力,采用Transwell实验检测各组胰腺癌细胞PANC-1和SW1990的侵袭能力,采用Western blot检测各组胰腺癌细胞PANC-1和SW1990的凋亡蛋白[半胱天冬酶-3(Caspase-3)、Caspase-9]和上皮细胞-间充质转化(EMT)蛋白[E-钙黏蛋白(E-cadherin)、N-cadherin及波形蛋白(Vimentin)]的表达。结果与DMSO组比,随着FKB浓度的增加,PANC-1和SW1990细胞的增殖和侵袭能力逐渐降低,差异均有统计学意义(P<0.05);与DMSO组比,随着FKB浓度的增加,PANC-1和SW1990细胞的Caspase-3、Caspase-9、E-cadherin蛋白表达增加,N-cadherin和Vimentin蛋白表达下降,差异均有统计学意义(P<0.05)。结论FKB可抑制人胰腺癌细胞的增殖及侵袭能力,机制可能与促进细胞凋亡、干预EMT过程有关。Objective To investigate the effect and mechanism of Flavokawain B(FKB)on the proliferation and invasion of human pancreatic cancer cells.Methods The human pancreatic cancer cell lines PANC-1 and SW1990 in the logarithmic growth phase were selected,and the control group[0.1%dimethyl sulfoxide(DMSO)]and 5,10,20,and 40μmol/L FKB groups were set up respectively.The proliferation effect of FKB on human pancreatic cancer cells was detected by Cell-Counting-Kit-8(CCK-8)and plate clone formation assay.The effect of FKB on the invasive ability of pancreatic cancer cells was detected by Transwell invasion assay.Western blot was used to detect the expression of apoptotic proteins(Caspase-3,Caspase-9)and epithelial-mesenchymal transition(EMT)proteins(E-cadherin,N-cadherin,and Vimentin)in PANC-1 and SW1990 cells.Results Compared with DMSO group,the proliferation and invasion ability of PANC-1 and SW1990 cells decreased with the increase of FKB concentration(P<0.05);Compared with DMSO group,with the increase of FKB concentration,the expressions of Caspase-3,Caspase-9,and E-cadherin in PANC-1 and SW1990 cells increased,while the expressions of N-cadherin and Vimentin decreased(P<0.05).Conclusion FKB can inhibit the proliferation and invasion of human pancreatic cancer cells,and the mechanism may be related to promoting apoptosis and interfering with EMT process.

关 键 词：胰腺肿瘤 细胞凋亡 细胞增殖 卡瓦胡椒素B 上皮细胞-间充质转化 侵袭 半胱天冬酶-3 

分 类 号：R735.9[医药卫生—肿瘤]