黑麦草生长指标及微生物多样性对锰渣-磷石膏混合基质中水泥含量的响应  

Responses of Ryegrass Growth Indexes and Microbial Diversity to Cement Content in Manganese Residue-Phosphogypsum Mixed Matrix

作　　者：代洁 刘方[1,2] 朱健[1,2] 罗海波[1] 敖慧[1] DAI Jie;LIU Fang;ZHU Jian;LUO Haibo;AO Hui(Department of Resources and Environmental Engineering,Guizhou University,Guiyang,Guizhou 550025;Key Laboratory of Karst Geological Resources and Environment of Ministry of Education,Guiyang,Guizhou 550025)

机构地区：[1]贵州大学资源与环境工程学院,贵州贵阳550025 [2]贵州喀斯特环境生态系统教育部野外科学观测研究站,贵州贵阳550025

出　　处：《北方园艺》2022年第12期86-93,共8页Northern Horticulture

基　　金：国家自然科学基金委员会-贵州省人民政府喀斯特科学研究中心资助项目(U1612442);国家自然科学基金资助项目(41661094);贵州省科技计划资助项目(黔科合平台人才[2017]5788号)。

摘　　要：以电解锰渣-磷石膏混合基质为试材,采用在不同水泥含量的电解锰渣-磷石膏混合基质中种植黑麦草的方法,研究了水泥含量对黑麦草生长指标及微生物多样性的影响,以期为电解锰渣-磷石膏混合基质应用于植被混凝土生态修复提供参考依据。结果表明:黑麦草株高、总生物量均随着水泥含量的升高而下降,在15%水泥含量下分别下降至对照的71.78%、72.12%。随着水泥含量(3%~15%)的增加,黑麦草的叶绿素含量呈先增加后降低的趋势,丙二醛(MDA)含量、抗氧化酶(SOD、CAT、POD)活性呈先降低后增加的趋势,水泥含量超过3%时,黑麦草生长受到抑制。水泥添加对黑麦草生长下电解锰渣-磷石膏混合基质门、属水平上优势菌群种类无明显影响,但其添加水泥后拟杆菌门相对丰度升高了70.71%~71.65%,Ohtaekwangia相对丰度升高了79.36%~165.50%。综合考虑黑麦草生长与微生物多样性,电解锰渣-磷石膏混合基质中水泥含量为不超过3%为宜,黑麦草具有作为电解锰渣-磷石膏混合基质应用于植被混凝土生态修复备选植物的潜力。Taking electrolytic manganese residue-phosphogypsum mixed matrix as the test material, adopting the method of planting ryegrass in the mixed matrix of electrolytic manganese residue and phosphogypsum with different cement contents, the effects of cement content on the growth and microbial diversity of ryegrass were studied, in order to provide reference for the application of electrolytic manganese residue-phosphogypsum mixed matrix to the ecological restoration of vegetation concrete.The results showed that the plant height and total biomass of ryegrass decreased with the increase of cement content, and decreased to 71.78% and 72.12% of the control respectively at 15% cement content.With the increase of cement content(3%-15%),the chlorophyll content of ryegrass showed a trend of increasing and then decreasing, the content of MDA and the activities of antioxidant enzymes(SOD,CAT,POD) showed a trend of decreasing and then increasing, when the cement content exceeds 3%,the growth of ryegrass was inhibited.The addition of cement had no significant effect on the dominant flora at the phylum and genus level of the electrolytic manganese residue-phosphogypsum mixed matrix under the growth of ryegrass, but the relative abundance of Bacteroidetes increased by 70.71%-71.65% after the addition of cement.Ohtaekwangia abundance increased by 79.36%-165.50%.Considering the growth and microbial diversity of ryegrass comprehensively, the cement content in the electrolytic manganese residue-phosphogypsum mixed matrix should be no more than 3%,and ryegrass could be used as the electrolytic manganese residue-phosphogypsum mixed matrix to be used as an alternative plant for ecological restoration of vegetation concrete potential.

关 键 词：电解锰渣 水泥 黑麦草 抗氧化酶 微生物多样性 

分 类 号：X757[环境科学与工程—环境工程]