Enhanced corrosion resistance,antibacterial activity and biocompatibility of gentamicin-montmorillonite coating on Mg alloy-in vitro and in vivo studies  

作　　者：Lijun Fan Wenxin Sun Yuhong Zou Qian-qian Xu Rong-Chang Zeng Jingrui Tian 

机构地区：[1]Department of Bioengineering,College of Chemical and Biological Engineering,Shandong University of Science and Technology,Qingdao 266590,China [2]Light Alloy Corrosion Laboratory,College of Materials Science and Engineering,Shandong University of Science and Technology,Qingdao 266590,China [3]Hospital of Shandong University of Science and Technology,Qingdao 266590,China [4]School of Materials Science and Engineering,Zhengzhou University,Zhengzhou 450002,China

出　　处：《Journal of Materials Science & Technology》2022年第16期167-180,共14页材料科学技术（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(No.52071191);the Natural Science Foundation of Shandong Province(No.ZR 2020ME011)。

摘　　要：Implant-associate infection(IAI)is a major cause of failure of bone implant materials,and one of the significant challenges in clinical managements.A synergistic coating strategy combining montmorillonite(MMT)sustained release,adsorption of bacteria and gentamicin(GS)bactericidal is proposed herein to tackle infection issues.Surface morphology,microstructure and chemical composition of the samples were investigated using SEM,XRD,FT-IR and XPS.Electrochemical experiments and immersion experiments reveal that corrosion resistance of Mg samples with GS/MMT coatings was higher than that of bare Mg alloy substrate in DMEM solution.In vitro studies demonstrated that the GS/MMT coating had a significant inhibitory effect on Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus).The viability of MC3T3-E1 cells was 92.7%after a co-culturing for 72 h.After a subcutaneous transplantation of 90days,the survival rate was 100%for GS/MMT-coated Mg alloy specimens with no infection at the implantation sites and no toxic damage to liver,kidney and local muscles pathological sections.This study provides a novel method for the preparation of sustained-release antimicrobial coatings on biodegradable Mg alloys as promising candidates for orthopedic implant materials.

关 键 词：Magnesium alloy MONTMORILLONITE GENTAMICIN Antibacterial activity COATING 

分 类 号：R318.08[医药卫生—生物医学工程] TG174.4[医药卫生—基础医学]