广西新型鸭呼肠孤病毒流行毒株GX01-2020全基因组分子特征分析  

Molecular characteristics of whole genome of novel duck reovirus GX01-2020 strain from Guangxi Province

作　　者：谢守玉 刘惠心 周媛 熊陈勇 施开创 屈素洁 苏文广 温新瑞 李媛 李春英 邓桂潮 尹彦文 XIE Shouyu;LIU Huixin;ZHOU Yuan;XIONG Chenyong;SHI Kaichuang;QU Sujie;SU Wenguang;WEN Xinrui;LI Yuan;LI Chunying;DENG Guichao;YIN Yanwen(Guangxi Center for Animal Disease Control and Prevention,Nanning 530001 China;College of Animal Science and Technology,Guangxi University,Nanning 530005,China;Beihai Center for Animal Disease Control and Prevention,Beihai,Guangxi 536000,China;Qinzhou Center for Animal Disease Control and Prevention,Qinzhou,Guangxi 535099,China;Pubei Town of Qinzhou Center for Animal Disease Control and Prevention,Qinzhou,Guangxi 535300,China)

机构地区：[1]广西壮族自治区动物疫病预防控制中心,广西南宁530001 [2]广西大学动物科学技术学院,广西南宁530005 [3]北海市动物疫病预防控制中心,广西北海536000 [4]钦州市动物疫病预防控制中心,广西钦州535099 [5]浦北县动物疫病预防控制中心,广西钦州535300

出　　处：《中国兽医学报》2022年第4期643-650,661,共9页Chinese Journal of Veterinary Science

基　　金：广西自然科学基金资助项目(2020GXNSFBA297066);广西农业科技基金资助项目(Z201984,Z202032)。

摘　　要：为了解广西新型鸭呼肠孤病毒(novel duck reovirus,NDRV)流行毒株的分子遗传特征,采用RT-PCR分段进行全基因组序列扩增,经克隆、测序、拼接后,获得1株NDRV全基因组序列(命名为GX01-2020株)。结果显示,GX01-2020株基因组全长23353 bp,分为10个片段,大小从1194 bp(S4)至3958 bp(L1)不等。同源性分析显示,GX01-2020株10个片段核苷酸同源性与NDRV代表毒株最高,为86.2%~99.4%,其中μB片段核苷酸同源性最低的毒株为番鸭呼肠孤病毒(Muscovy duck reovirus,MDRV),其他片段则为鸡源禽呼肠孤病毒(chicken-origin reovirus,CRV),说明不同片段来源祖先不完全相同。遗传进化分析显示,10个片段遗传进化树中,L2、L3片段中MDRV参考毒株及σA片段中NDRV参考毒株均形成两个进化分支,与其他片段不同,说明不同片段与参考毒株的遗传关系不尽相同。重组分析显示,GX01-2020株的L1片段存在重组信号,重组亲本毒株分别为北京MDRV_J18株和福建DRV_NP03株。本研究丰富了NDRV基因库相关信息,同时为广西地区NDRV的分子流行病学研究和有效防制措施制订提供了一定的参考。To investigate the molecular characteristics of a novel duck reovirus(NDRV)from Guangxi Province,the genomic segments of NDRV were cloned from positive samples by RT-PCR,then they were sequenced,the complete genome sequence of a NDRV strain was obtained after splicing,the strain was named GX01-2020.The genome length of the GX01-2020 strain was 23353 bp containing 10 segments including L1-L4,M1-M3 and S1-S4.The size of these fragments varies from 1194 bp(S4)to 3958 bp(L1).Homology analysis of L,M and S segments of GX01-2020 revealed that the highest nucleotide identity to the NDRV reference strains was 86.2%-99.4%,andμB segment has the lowest nucleotide identity with MDRV(Muscovy duck reovirus).The other segments share similar sequences to CRV(chicken-origin reovirus),indicating that the ancestors of all segments were not identical.Phylogenetic analysis using L2and L3for MDRV strains andσA for NDRV reference strains indicated that they belonged to two different branches,respectively,which were different from the results analyzed by using other segments,suggesting that genetic distance of 10segments with reference strains was not the same.The recombination signal of L1segment was detected with RDP4and SimPlot software,the results indicated that the major and minor parents of recombination were classical reference strains MDRV_J18of Peking and DRV_NP03of Fujian Province.Those results will enrich genetic information of NDRV,and provide basis for research on molecular epidemiology and strategy of control and prevention for NDRV in Guangxi Province.

关 键 词：新型鸭呼肠孤病毒(NDRV) 全基因组测序 遗传进化 

分 类 号：S852.65[农业科学—基础兽医学]