Progress,problems and prospects of porcine pluripotent stem cells  

作　　者：Hanning WANG Yangli PEI Ning LI Jianyong HAN 

机构地区：[1]State Key Laboratory for Agrobiotechnology,College of Biological Sciences,China Agricultural University,Beijing 100193,China

出　　处：《Frontiers of Agricultural Science and Engineering》2014年第1期6-15,共10页农业科学与工程前沿（英文版）

基　　金：This work was funded by the National Key Basic Research and Development Program of China(2010CB945404,2011CBA01001 and 2009CB941003);the Chinese Universities Scientific Fund(2012RC014);the National Thousand Talents Program of China.

摘　　要：Pluripotent stem cells(PSCs),including embryonic stem cells(ESCs)and induced PSCs(iPSCs),can differentiate into cells of the three germ layers,suggesting that PSCs have great potential for basic developmental biology research and wide applications for clinical medicine.Genuine ESCs and iPSCs have been derived from mice and rats,but not from livestock such as the pig-an ideal animal model for studying human disease and regenerative medicine due to similarities with human physiologic processes.Efforts to derive porcine ESCs and iPSCs have not yielded high-quality PSCs that can produce chimeras with germline transmission.Thus,exploration of the unique porcine gene regulation network of preimplantation embryonic development may permit optimization of in vitro culture systems for raising porcine PSCs.Here we summarize the recent progress in porcine PSC generation as well as the problems encountered during this progress and we depict prospects for generating porcine naive PSCs.

关 键 词：induced pluripotent stem cells embryonic stem cells PIG REPROGRAMMING 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]