The microRNA,miR-29c,participates in muscle development through targeting the YY1 gene and is associated with postmortem muscle pH in pigs  

作　　者：Weiya ZHANG Wei WEI Yuanyuan ZHAO Shuhong ZHAO Xinyun LI 

机构地区：[1]Key Lab of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education&Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China [2]College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Frontiers of Agricultural Science and Engineering》2015年第4期311-317,共7页农业科学与工程前沿（英文版）

基　　金：This work was supported by the National Outstanding Youth Foundation of NSFC(31025026);National Basic Research Program of China(2012CB124702);New Breed Creation Using Transgenic Methods(2014ZX0800950B).

摘　　要：Previous studies indicated that miR-29c is important for muscle development in mice and human,but its role in pigs is unknown.In this study,we detected the expression of miR-29c in Meishan longissimus lumborum(LL)muscle.The results showed that miR-29c was gradually upregulated during development of skeletal muscle in pig.Moreover,the expression of YY1 and Akt3 genes,which were confirmed to be targeted by miR-29c in mice,was decreased along with muscle development.Furthermore,the expression level of miR-29c was significantly higher in adult Meishan pigs than Large White pigs,while the expression of YY1 and Akt3 genes was significantly lower in Meishan pigs.These results indicated that the expression pattern of miR-29c was opposite to that of YY1 and Akt3 genes in pigs.Also,the luciferase assay indicated that miR-29s can target the YY1 gene in pigs.In addition,we identified a T to C mutation in the primary transcript of miR-29c,which was associated with the postmortem muscle pH in pigs.Based on these results,we concluded that miR-29c is also important in skeletal muscle development of pigs.

关 键 词：PIG miR-29c skeletal muscle expression SNP 

分 类 号：R73[医药卫生—肿瘤]