2D-LC在中药分析与质量控制中的应用进展  

Application Progress of 2D-LC in Analysis and Quality Control of Traditional Chinese Medicine

作　　者：叶楚璇 黄文静 覃思意[1] 麦子盈 王乐琪 李莎莎 徐浩然 王登辉 严诗楷 肖雪 YE Chu-xuan;HUANG Wen-jing;QIN Si-yi;MAI Zi-ying;WANG Le-qi;LI Sha-sha;XU Hao-ran;WANG Deng-hui;YAN Shi-kai;XIAO Xue(Guangdong Metabolic Disease Research Center of Integrated Chinese and Western Medicine,Institute of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;NMPA Key Laboratory for Rapid Testing Technology of Drugs,Guangdong Institute for Drug Control,Guangzhou 510663,China;The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China;School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]广东药科大学中医药研究院广东省代谢病中西医结合研究中心,广东广州510006 [2]国家药品监督管理局药品快速检验技术重点实验室(广东省药品检验所),广东广州510663 [3]广州中医药大学第二附属医院,广东广州510120 [4]上海交通大学药学院,上海200240

出　　处：《化学试剂》2022年第8期1103-1110,共8页Chemical Reagents

基　　金：国家自然科学基金资助项目(81830117,81603070);国家药品监督管理局快速检验技术重点实验室开放课题项目(KF2022002,KF2022006);广东省农业农村厅省级乡村振兴战略专项农业科技创新及推广体系建设项目(2020KJ265);重庆市重点实验室开放课题项目(ZDSYS-KF-2021-1)。

摘　　要：中药质量控制是保证其药效及安全的关键因素,也是中医药产业良性发展的重要保障。高效液相色谱技术作为一种经典、高效的检测技术,具有强大的分离效能,能够对中药中复杂成分进行有效分离及含量测定,已广泛应用于中药的质量控制中。随着液相技术的发展,二维液相色谱技术的出现弥补了一维液相色谱技术中样品前处理复杂,基体干扰较大等缺点,其灵敏度更高,分离度更好,能够快速,准确对中药做出分析、评价及质量控制。二维液相色谱技术因其普适性与简便性,使得中药分析更加全面、更加完善。从二维液相色谱技术在中药化学成分的系统分离、全面定性、精准定量、风险物质检测等多个方面,阐述二维液相色谱技术在中药分析及质量控制中发挥的作用。Quality control of traditional Chinese medicine(TCM)is the key factor to ensuring its efficacy and safety,and also an important guarantee for the continuous development of the traditional Chinese medicine industry.As a classic and efficient detection technology,high-performance liquid chromatography(HPLC)has been widely used in the quality control of traditional Chinese medicine because of its powerful separation skills and effective separation and content determination of complex components in traditional Chinese medicine.With the development of liquid phase technology,the appearance of two-dimensional liquid chromatography technology(2D-LC)has made up for the shortcomings of one-dimensional liquid chromatography technology,such as complex sample pretreatment,large matrix interference,and so on.Its sensitivity is higher,the separation degree is better,and it can quickly and accurately evaluate and control the quality of traditional Chinese medicine.Due to its universality and simplicity,2D-LC makes qualitative and quantitative analysis of TCM more comprehensive.This paper aims to expound on the role of 2D-LC in the analysis and quality control of traditional Chinese medicine from the following aspects,such as the overall characterization,the study of chemical composition,the quantitative analysis of complex components,and the control of risk substances,etc.

关 键 词：二维液相色谱 中药分析 质量控制 应用进展 

分 类 号：R917[医药卫生—药物分析学]