桑叶水提物改善肠肌运动及缓解便秘的作用  

Effect of Mulberry Leaf Water Extract on Improving Intestinal Muscle Movement and Relieving Constipation

作　　者：段晓峰 王亚文 耿旭芳 赵甜 李丽 贾东升[2] 赵丁[1] DUAN Xiaofeng;WANG Yawen;GENG Xufang;ZHAO Tian;LI Li;JIA Dongsheng;ZHAO Ding(College of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China;Institute of Cash Crops of Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China)

机构地区：[1]河北医科大学药学院,河北石家庄050017 [2]河北省农林科学院经济作物研究所,河北石家庄050051

出　　处：《食品工业科技》2022年第15期344-351,共8页Science and Technology of Food Industry

基　　金：河北省重点研发计划项目(20327121D);河北省科技厅-河北医科大学“厅校会商基金-科技创新”项目(2020TXZH05);河北省农林科学院科技创新工程项目(2019-2-3-01)。

摘　　要：目的:研究桑叶水提物对豚鼠离体回肠收缩活动及对便秘大鼠的影响,探讨其改善便秘的作用及相关机制。方法:通过离体肠肌张力测定手段,观察桑叶水提物对豚鼠离体回肠运动的影响。采用洛哌丁胺(10 mg/kg/d)构建3 d及7 d便秘大鼠模型,并以桑叶水提物(5 g/kg/d)同期灌胃给药进行干预,观察大鼠第一白便时间、24 h粪便粒数和小肠推进率等,以及卡巴胆碱(carbachol,CCh)诱发各组大鼠回肠收缩反应的情况;通过石蜡切片HE染色观察大鼠结肠组织形态学变化,免疫组化检测结肠组织水通道蛋白3(aquaporin 3,AQP3)的表达。结果:桑叶水提物能促进豚鼠回肠的自主运动,表现为收缩幅度增强,平均张力升高。桑叶水提物对造模3 d和造膜7 d大鼠的第一白便时间、24 h粪便粒数(造模7 d,P<0.05)及小肠推进率(造模7 d,P<0.05)等指标均有不同程度改善;桑叶水提物明显改善卡巴胆碱诱发的便秘大鼠离体回肠平均张力的降低(P<0.05);桑叶水提物对洛哌丁胺引起的大鼠结肠组织肌层变薄有改善作用,并显著降低洛哌丁胺引起的结肠AQP3表达的增加(P<0.05)。结论:桑叶水提物明显促进豚鼠回肠运动,并可能通过降低AQP3的表达,抑制结肠水分过度吸收,使粪便含水量增加,促进肠肌运动而改善便秘。Objective:To study the effect of mulberry leaf water extract on isolated ileum contractile activity of guinea pig and constipation rats,and to explore the mulberry leaf improving constipation effects and related mechanisms.Methods:The effect of mulberry leaf water extract on isolated ileum movement of guinea pigs was observed by measuring in vitro intestinal muscle tension.Loperamide(10 mg/kg/d)was used to construct 3 d and 7 d constipation rat models,and mulberry leaf water extract(5 g/kg/d)was administered by gavage at the same time to observe the time of first defecation,fecal number for 24 h and small intestine propulsion rate of rats.The ileum contractile reaction induced by carbachol was measured.The histological changes of colon were observed by HE staining of paraffin section,and the expression of AQP3 in colon was detected by immunohistochemistry.Results:Mulberry leaf water extract promoted the voluntary movement of ileum in guinea pigs,which was manifested by increased contraction amplitude and mean tension.The first defecation time,fecal number at 24 h and small intestine propulsion rate were improved in both group of mulberry leaf 3 d and 7 d.Mulberry leaf water extract significantly improved the reduction of the average tension of the isolated ileum induced by carbachol in constipation rats.Mulberry leaf water extract improved the musculature thinning of colon tissue caused by loperamide,and significantly reduced the increase in colon AQP3 expression casued by loperamide.Conclusion:Mulberry leaf water extract can significantly promote ileum movement in guinea pigs,and improve constipation in rats,which may through reducing the expression of AQP3,inhibiting the excessive absorption of colonic water,increasing the water content of feces,and promoting intestinal muscle movement.

关 键 词：桑叶水提物 豚鼠 肠肌运动 便秘大鼠 水通道蛋白3(AQP3) 结肠 

分 类 号：R285.5[医药卫生—中药学]