核桃浆对鼠李糖乳杆菌培养特性及其富硒后抗缺氧活性的影响  

Effects of Walnut Pulp on the Cultivation Characteristics and Antihypoxia Potential of Lactobacillus rhamnosus Enriched with Selenium

作　　者：李颖慧 徐枫钦 刘冠闻 曲文娟[2] 马海乐[2] Aniqa ZIA 师俊玲[1] LI Yinghui;XU Fengqin;LIU Guanwen;QU Wenjuan;MA Haile;Aniqa ZIA;SHI Junling(School of Life Sciences,Northwestern Polytechnical University,Xi’an 710072,China;Institute of Food Physical Processing,Jiangsu University,Zhenjiang 212013,China)

机构地区：[1]西北工业大学生命学院,陕西西安710072 [2]江苏大学食品物理加工研究院,江苏镇江212013

出　　处：《食品工业科技》2022年第14期127-137,共11页Science and Technology of Food Industry

基　　金：国家重点研发计划政府间国际科技创新合作重点专项(2017YFE0105300)。

摘　　要：为考察以核桃浆为原料开发廉价、高效的鼠李糖乳杆菌培养体系的可行性,本文用核桃浆代替MRS培养基中氮源,研究了核桃浆的颗粒度、料液比、用量、脱脂处理以及摇床转速对鼠李糖乳杆菌的生长、发酵液抗菌活性和抗氧化活性的影响,并用所得菌体经富硒处理后饲喂小鼠3 d,监测小鼠在暴露于低压缺氧条件下(海拔5000 m)6 h后的血细胞和血红蛋白含量,评价其抗缺氧潜力。结果发现,采用料液比为1:10(g/mL),经120目筛网过滤的去脂核桃浆完全替代MRS培养基中氮源(牛肉膏、蛋白胨),可使培养基成本降低70%,使菌体产量提高2.5倍(从2.6×10^(9)至7.6×10^(9) CFU/mL),但不影响发酵液的抗菌活性和抗氧化活性(P>0.05)。连续口服富硒菌体3 d,可显著提高小鼠在低压缺氧条件下的血红蛋白含量(P<0.05),优于MRS培养基所得菌体。综上,用核桃浆能够完全代替MRS培养基中氮源用于培养鼠李糖乳杆菌,并能有效提高菌体产量和活性。In order to develop a cheap and efficient culture system of Lactobacillus rhamnosus,walnut pulp was used to replace the nitrogen source in MRS medium.Taking cell growth as the indicator,the cultivation system was optimized in aspects of walnut pulp particle size,solid-liquid ratio,dosage,lipid removing treatment,and the shaking speed during the cultivation of Lactobacillus rhamnosus.The antibacterial activity and antioxidant activity of cell-free culture supernatant were also tested in all conditions.The obtained cells were also subjected to selenium enrichment treatment and then fed to mice for 3 d before they were exposed to simulated low-pressure hypoxia condition(altitude 5000 m)for 6 h to evaluate their anti-hypoxia potential.As results,complete replacement of the nitrogen source(beef extract and peptone)of MRS medium with the lipid-free walnut pulp filtered by 120 mesh screen at the solid-liquid ratio of 1:10(g/mL)could reduce the cost of MRS medium by 70%and increase the cell yield by 2.5 times(from 2.6×10^(9) to 7.6×10^(9) CFU/mL),but did not affect the antibacterial activity and antioxidant activity of the fermentation broth(P>0.05).Continuous oral administration of selenium enriched bacteria for 3 days could significantly increase the hemoglobin content of mice when they were exposed to low-pressure hypoxia for 6 h(P<0.05),showing better effects than that cultivated in MRS medium.In conclusion,walnut pulp could be used to replace the nitrogen source in MRS medium for the culture of Lactobacillus rhamnosus,and effectively improve the cell growth and activity.

关 键 词：鼠李糖乳杆菌 富硒菌 核桃 氮源 液体培养 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]