Treg/Th17细胞免疫平衡紊乱与急性胰腺炎疾病进展相关性研究  

Correlation between Treg/Th17 cell immune balance disorder and disease progression of acute pancreatitis

作　　者：张涛[1] 李智伟[2] 徐辉[1] ZHANG Tao;LI Zhiwei;XU Hui(Department of Pancreatic Surgery,People’s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,P.R.China;Department of Clinical Laboratory Center,People’s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,P.R.China)

机构地区：[1]新疆维吾尔自治区人民医院胰腺外科,乌鲁木齐830001 [2]新疆维吾尔自治区人民医院临床检验中心,乌鲁木齐830001

出　　处：《中国普外基础与临床杂志》2022年第7期892-896,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：新疆维吾尔自治区自然科学基金(项目编号:2018D01C138);新疆维吾尔自治区人民医院科技创新引进创新项目(项目编号:20180102)。

摘　　要：目的分析调节性T细胞(regulatory T cell,Treg)/辅助性T细胞17(T helper 17 cell,Th17)失衡与急性胰腺炎(acute pancreatitis,AP)发病机制之间的关系,探讨AP患者Treg/Th17细胞失衡与辅助性T细胞1和辅助性T细胞2及细胞因子之间的关系,为AP的免疫治疗提供新的治疗靶点。方法选取2020年1–12月期间在新疆维吾尔自治区人民医院就诊并明确诊断为AP的患者40例(AP组)以及同期行健康体检者40例(正常对照组)作为研究对象,采集其外周血,用流式细胞仪检测Treg和Th17细胞的比例;检测血浆白细胞介素(interleukin,IL)-10和IL-17的水平。结果与正常对照组相比,AP组治疗前的Treg和Th17细胞比例均增高,差异有统计学意义(t=5.78、P<0.001,t=5.82、P<0.001),IL-10和IL-17水平均增高,差异有统计学意义(t=7.14、P<0.001,t=35.22、P<0.001);AP组治疗后与正常对照组相比,Treg和Th17细胞比例均有增高但差异无统计学意义(t=1.87、P>0.05,t=0.29、P>0.05),IL-10水平增高,差异有统计学意义(t=3.98,P<0.001),IL-17水平增高但差异无统计学意义(t=1.67,P>0.05);AP组治疗后与治疗前相比,Treg和Th17细胞比例均下降,差异有统计学意义(t=3.07、P<0.01,t=4.99、P<0.001),IL-10和IL-17水平也均下降,差异有统计学意义(t=3.38、P<0.001,t=30.63、P<0.001)。结论AP时,Treg细胞介导免疫抑制,Th17细胞介导炎症反应,促进疾病的炎症发生发展。IL-10与IL-17可能在调节两者的分化和平衡过程中起重要作用。Objective To analyze the relation between regulatory T cell(Treg)/helper T cell 17(Th17)imbalance and the pathogenesis of acute pancreatitis(AP)and to explore the relation between Treg/Th17 cell imbalance and helper T cells 1,helper T cells 2 and cytokines in patients with AP,so as to provide a new therapeutic target for immunotherapy of AP.Methods From January to December 2020,40 patients diagnosed with AP(AP group)in The People’s Hospital of Xinjiang Uygur Autonomous Region and 40 healthy subjects who underwent physical examination(normal control group)during the same period in this hospital were selected as the research objects.Their peripheral bloods were collected and the proportion of Treg and Th17 cells was detected by flow cytometry.Plasma levels of interleukin-10(IL-10)and interleukin-17(IL-17)were detected.Results Compared with the normal control group,the proportions of Treg and Th17 cells increased before treatment in the AP group,the differences were statistically significant(t=5.78,P<0.001;t=5.82,P<0.001).The levels of IL-10 and IL-17 increased,the differences were statistically significant(t=7.14,P<0.001;t=35.22,P<0.001).After treatment,the AP group as compared with the normal control group,the proportions of Treg and Th17 cells increased but the differences were not statistically significant(t=1.87,P>0.05;t=0.29,P>0.05),the level of IL-10 increased and the difference was statistically significant(t=3.98,P<0.001),the level of IL-17 increased but the difference was not statistically significant(t=1.67,P>0.05).After treatment as compared with before treatment in the AP group,the proportions of Treg and Th17 cells decreased,the differences were statistically significant(t=3.07,P<0.01;t=4.99,P<0.001).The levels of IL-10 and IL-17 decreased,the differences were statistically significant(t=3.38,P<0.001;t=30.63,P<0.001).Conclusion In AP,Treg cells mediate immunosuppression and Th17 cells mediate inflammatory response,promoting the occurrence and development of inflammation in the disease.IL-10 and IL-17

关 键 词：急性胰腺炎 调节性T细胞 辅助性T细胞17 白细胞介素-10 白细胞介素-17 

分 类 号：R576[医药卫生—消化系统]