洋甘菊总黄酮对细胞脂质代谢的作用及其机制研究  

Effect and mechanism of total flavonoids of chamomile on lipid metabolism in cells

作　　者：兰卫[1] LAN Wei(College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830017)

机构地区：[1]新疆医科大学中医学院,乌鲁木齐830017

出　　处：《中国食品添加剂》2022年第8期8-17,共10页China Food Additives

基　　金：国家自然科学地区基金项目(81960771);国家重点研发计划项目(2017YFC1703902-3);新疆维吾尔自治区自然科学基金资助项目(No.2018D01C160);2018年全国中药特色技术传承人才培训项目(T20184828005);第四次全国中药资源普查阿克苏地区项目(GZY-KJS-2018-004)。

摘　　要：目的:考察洋甘菊总黄酮降脂作用,并基于PPARa(过氧化物酶体增殖物激活受体)信号通路探究其作用机制。方法:体外实验选用正常肝癌细胞(HepG2),采用脂肪酸溶液诱导细胞脂肪变性,用不同剂量的洋甘菊总黄酮(100、150、200mg/mL)和非诺贝特(10mmol/L)处理24h后,用油红O染色观察细胞内脂滴,测定细胞内TG含量;Western-blot法检测肝细胞内脂代谢相关蛋白PPARa、CPT1A(肉碱脂酰转移酶1A)和Acox1(酰基铺酶A氧化酶)的表达水平。结果:洋甘菊总黄酮能显著上调HepG2细胞PPARa、CPT1A和Acox1蛋白的表达,并抑制细胞内TG的合成,显著改善细胞内脂滴蓄积。结论:洋甘菊总黄酮通过上调PPARa、CPT1A和Acox1蛋白的表达,促使脂肪酸氧化,进而发挥降血脂作用和抗肝脏脂变作用。Objective:To investigate the lipid-lowering effect of total flavonoids from chamomile and explore its mechanism based on PPARa signaling pathway.Methods:Normal liver cancer cells(HepG2)were selected in the in vitro experiment.Fatty acid solution was used to induce cell steatosis.After 24 h of treatment with different doses of total flavonoids of chamomile(100,150,200mg/mL)and fenofibrate(10mmol/L),oil red O staining was used to observe intracellular lipid droplets and determine intracellular TG content.The expression levels of PPARa,CPT1A and Acox1 in hepatocytes were detected by Western-blot.Results:Total flavonoids of chamomile could significantly up-regulate the expression of PPARa,CPT1A and Acox1 proteins in HepG2 cells,inhibit the synthesis of intracellular TG,and significantly improve the accumulation of intracellular lipid droplets.Conclusion:Total flavonoids of chamomile promote the oxidation of fatty acids by up-regulating the expression of PPARa,CPT1A and Acox1 proteins,and thus plays a role in lowering blood lipids and of anti-hepatic steatosis.

关 键 词：洋甘菊总黄酮 高脂血症 HEPG2人肝癌细胞 

分 类 号：TS202.1[轻工技术与工程—食品科学]