小鼠海马NONO基因沉默对焦虑样行为的影响  

The effects of NONO gene silencing on the anxiety-like behavior of mice

作　　者：冯莹[1] 方志成[1] 陈伟[1] 郑翔[1] Feng Ying;Fang Zhicheng;Chen Wei;Zheng Xiang(Department of Intensive Care Unit,Taihe Hospital of Shiyan,Shiyan 442000,China)

机构地区：[1]十堰市太和医院,重症医学科,十堰442000

出　　处：《神经解剖学杂志》2022年第3期307-313,共7页Chinese Journal of Neuroanatomy

基　　金：湖北省卫健委面上项目(WJ2019M059);十堰市科技局指导项目(19Y32,20Y28)。

摘　　要：目的:探索小鼠海马区不含POU结构的八聚核苷酸结合蛋白(NONO)基因沉默后突触相关基因的表达及行为学变化。方法:构建并包装表达NONO基因靶向shRNA的重组慢病毒(LV-NONO),海马区显微注射重组慢病毒干扰NONO基因的表达,实验小鼠分为对照组(control),对照病毒组(LV-Con)和NONO慢病毒干扰组(LV-NONO)。慢病毒感染5 d检测海马NONO的表达,14 d检测海马γ-氨基丁酸A型受体α2亚基(GABA)和桥尾蛋白(gephyrin)的表达,旷场实验和O迷宫实验检测实验小鼠的行为学变化。结果:LV-NONO组小鼠海马区NONO、GABA和gephyrin的表达显著低于对照组小鼠。旷场实验和O迷宫实验结果显示,LV-NONO组小鼠进入开放臂的次数和中央区(开放臂)停留时间显著低于对照组小鼠。结论:海马区注射NONO干扰病毒抑制NONO基因的表达可以下调GABA和gephyrin的表达,进而增强小鼠焦虑样行为。Objective: To explore the expression of synapse-related genes and behavioral changes after NONO gene silencing in mouse hippocampus. Methods: Recombinant lentivirus expressing NONO gene targeting shRNA(LV-NONO) was constructed and packaged. The expression of NONO gene was interfered by microinjection of lentivirus into the hippocampus. The mice were divided into control group(control), control virus group(LV-Con) and NONO shRNA group(LV-NONO). The expression of NONO in the hippocampus was detected on the 5 th day after lentivirus infection, and the expressions of GABAand gephyrin in the hippocampus were detected on the 14 th day after lentivirus infection. The open field test and O maze test were used to detect the behavioral changes. Results: The expression of NONO, GABAand gephyrin in hippocampus of LV-NONO group was significantly lower than that of control group. The results of open field test and O maze test showed that the frequency of entering the open arm and the residence time in the central region(open arm) of LV-NONO group were significantly lower than those of control group. Conclusion: Inhibition of NONO expression in hippocampus can down-regulate the expression of GABAand gephyrin to enhance the anxiety-like behavior.

关 键 词：不含POU结构的八聚核苷酸结合蛋白(NONO) γ-氨基丁酸A型受体α2亚基GABA 桥尾蛋白 焦虑 小鼠 

分 类 号：R749.72[医药卫生—神经病学与精神病学]