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作 者:杨正嘉 张磊[2] 田野[2] 于克东 YANG Zheng-jia;ZHANG Lei;TIAN Ye;YU Ke-dong(Department of Thoracie Surgery,Liaoning Jinqiu Hospital,Shenyang 110016;Department of Thoracic Surgery,the Fourth Afiliated Hospital of China Medical University,Shenyang 110032,China)
机构地区:[1]辽宁省金秋医院胸外科,辽宁沈阳110016 [2]中国医科大学附属第四医院胸外科,辽宁沈阳110032
出 处:《解剖科学进展》2022年第2期161-164,168,共5页Progress of Anatomical Sciences
基 金:辽宁省自然科学基金(2018011490-301)。
摘 要:目的 探究miR-183-5p在肺癌中的表达及其通过靶向ZHX2对非小细胞肺癌细胞增殖及迁移的影响。方法 运用qRT-PCR检测miR-183-5p及ZHX2在20份配对肺癌样本的表达;应用荧光素酶报告系统验证miR-183-5p对ZHX2的靶向结合;构建miR-183-5p-mimics、miR-183-5p-inhibitor及pcDNA3.1-ZHX2慢病毒载体并转染细胞,qRT-PCR及Western blot方法检测ZHX2的表达;CCK8方法检测细胞增殖活力;Transwell实验检测细胞迁移能力;流式细胞术检测细胞凋亡。结果 与正常肺组织相比,miR-183-5p在肺癌组织中高表达(P<0.05),ZHX2在肺癌组织中低表达(P<0.05)。荧光素酶检测实验发现ZHX2是miR-183-5p的作用靶点。过表达miR-183-5p可抑制ZHX2的表达(P<0.05),抑制miR-183-5p可提高ZHX2的表达(P<0.05)。过表达miR-183-5p促进非小细胞肺癌细胞增殖与迁移(P<0.05),抑制凋亡(P<0.05);共转染pcDNA3.1-ZHX2可逆转这些作用。结论 miR-183-5p促进非小细胞肺癌细胞的增殖与迁移并抑制细胞凋亡,与靶向调控ZHX2有关。Objective To explore the expression of miR-183-5p in lung cancer and its effect on the proliferation and migration of non-small cell lung cancer cells by targeting ZHX2. Methods qRT-PCR was used to detect the expression of miR-183-5p and ZHX2 in 20 matched lung cancer samples. The luciferase reporter system was used to verify the targeted binding of miR-183-5p to ZHX2. miR-183-5p-mimics, miR-183-5p-inhibitor and PCDNA3.1-ZHx2lentivirus vectors were constructed and transfected into cells, and the expression of ZHX2 was detected by qRT-PCR and Western blot. Cell proliferation activity was detected by CCK8 method. Transwell assay was used to detect cell migration.Apoptosis was detected by flow cytometry. Results Compared with normal lung tissues, miR-183-5p was highly expressed in lung cancer tissues(P<0.05), and ZHX2 was low expressed in lung cancer tissues(P <0.05). Luciferase assay revealed that ZHX2 was the target of miR-183-5p. Overexpression of miR-183-5p could inhibit the expression of ZHX2(P<0.05), and inhibition of miR-183-5p could increase the expression of ZHX2(P<0.05). Overexpression of miR-183-5p promoted proliferation and migration of NSCLC cells(P<0.05) and inhibited apoptosis(P<0.05). These effects were reversed by cotransfection of PCDNA3.1-ZHx2. Conclusion miR-183-5p promotes proliferation and migration of non-small cell lung cancer cells and inhibits apoptosis, which is related to the targeted regulation of ZHX2.
关 键 词:miR-183-5p 肺癌 迁移 ZHX2
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