茶皂素抑制慢性系统性炎症诱导的小鼠脑内补体C3表达上调  

Inhibition of teasaponin on upregulation of complement component 3 in mice brain induced by chronic systemic inflammation

作　　者：张熠超 孙凯丽 谷莉 宋丹[1] ZHANG Yi-chao;SUN Kai-li;GU Li;SONG Dan(Central Laboratory,School of Pharmacy,China Medical Univerisity,Shenyang 110122,China;Department of Pharmacognosy,School of Pharmacy,China Medical Univerisity,Shenyang 110122,China)

机构地区：[1]中国医科大学药学院中心实验室,辽宁沈阳110122 [2]中国医科大学药学院中药与生药学教研室,辽宁沈阳110122

出　　处：《解剖科学进展》2022年第2期173-176,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(20180550571)。

摘　　要：目的 研究茶皂素对慢性系统性炎症诱导的小鼠脑内小胶质细胞激活和补体C3表达的影响。方法将小鼠随机分为对照组、脂多糖(LPS)组、茶皂素(TS)组和LPS+TS组。应用免疫荧光染色法观察小鼠脑内Iba-1阳性细胞数量;ELISA和荧光定量PCR法检测补体C3蛋白水平及mRNA表达的变化。结果 LPS给药后可引起外周血、脑皮层C3水平迅速增高, LPS停药后14d小鼠前额皮质Iba-1阳性细胞数量及补体C3蛋白和mRNA表达增加,发生时间较外周C3水平变化延后。而茶皂素预处理对LPS诱导的小鼠前额皮质Iba-1阳性细胞数量及补体C3蛋白和mRNA表达增加有明显的抑制作用。结论 茶皂素抑制LPS诱导的神经炎症作用与抑制脑内补体C3mRNA表达相关。Objective To investigate the effects of teasaponin on the activation of microglia and expression of complement component 3 in mice brain induced by chronic systemic inflammation. Methods Mice were randomly divided into the control group, LPS group, teasaponin group and LPS+teasaponin group. The number of Iba-1 positive cells was counted after immunofluorescence staining. The expression of C3 protein and mRNA was detected by ELISA and RT-qPCR,respectively. Results Systemic injection of low-dose LPS resulted in rapid increase of C3 in the periphery blood. Number of Iba-1 positive cells in frontal cortex, levels of C3 protein and mRNA were significantly increased at 14 days after cessation of LPS injection, which occurred later than the changes of systemic C3. Teasaponin treatment inhibited the increased number of Iba-1 positive cells and upregulation of C3 mRNA expression in frontal cortex induced by LPS. Conclusion The inhibitive effect of teasaponin on neuroinflammation induced by LPS is related to downregulation of C3 expression.

关 键 词：茶皂素 脂多糖 补体蛋白C3 小胶质细胞 慢性系统性炎症 小鼠 

分 类 号：R741.02[医药卫生—神经病学与精神病学]