基于多元统计分析的山银花抗氧化与抑菌质量标志物预测  

Prediction of antioxidant and antibacterial quality markers of Flos lonicerae based on multivariate statistical analysis

作　　者：王志辉[1,2] 周新茹 成惠珍 王智 童巧珍[1,2] 周日宝 刘湘丹[1,2,3] WANG Zhihui;ZHOU Xinru;CHENG Huizhen;WANG Zhi;TONG Qiaozhen;ZHOU Ribao;LIU Xiangdan(College of Pharmacy,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Key Research Office of Germplasm Resources and Standardized Planting of Medicinal Materials of Hunan Province,Changsha,Hunan 410208,China;Key Laboratory of Modern Research of TCM,Education Department of Hunan Province,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]湘产大宗道地药材种质资源及规范化种植重点研究室,湖南长沙410208 [3]湖南省普通高等学校中药现代化研究重点实验室,湖南长沙410208

出　　处：《湖南中医药大学学报》2022年第7期1105-1111,共7页Journal of Hunan University of Chinese Medicine

基　　金：国家现代农业产业技术体系资助项目(CARS-21);湖南省现代农业产业技术体系建设项目;2022湖南省中医药科研计划项目(D2022138);湖南省自然科学基金项目(2021JJ30479,2021JJ30515);2020年湖南省一流专业建设点:中药资源与开发;2020中药学一流学科开放基金(2020ZYX02);湖南省中医药管理局湘产大宗道地药材种质资源及规范化种植重点实验室;湖南省科技创新平台与人才计划-科技特派员创新创业项目(2020NK4231)。

摘　　要：目的建立灰毡毛忍冬抗氧化和抑菌谱-效关系质量评价系统,初步筛选出与抗氧化和抑菌药效相关的质量标志物。方法收集湖南各产地灰毡毛忍冬样品共20份,采用HPLC建立指纹图谱,并测定各样品的抗氧化和抑菌效果,通过SPSS 23.0软件对药效指标和共有峰面积进行偏最小二乘法回归、灰色关联度和相关性分析。结果关联度法分析得出3、12号峰与抗氧化和抑菌均有很强的相关性,偏最小二乘法回归系数可知2、3号峰与抗氧化效果相关性强,2、3、5、7、9、13、17、18号峰成分与抑菌效果相关性强,由双变量相关性分析可知3号峰与抗氧化效果显著相关,4、11和12号峰与抑菌效果显著相关,经对照品指认3号峰为绿原酸,7号峰为咖啡酸,11号峰为异绿原酸B,12号峰为异绿原酸A,17号峰为灰毡毛忍冬皂苷甲,18号峰为川续断皂苷乙,2、5、9、13号峰成分有待进一步确认。结论多种分析方法分析了山银花抗氧化和抑菌的“谱-效”和“量-效”关系,结果一致,预测有机酸类为抗氧化药效的质量标志物,皂苷和有机酸类为抑菌的质量标志物。Objective To establish a quality evaluation system for the antioxidant and antibacterial spectrum-effect relationship of lonicera macranthoides,and preliminarily screen the quality markers related to antioxidant and antibacterial.Methods A total of 20 samples of lonicera macranthoides from various places in Hunan were collected.HPLC fingerprints were established,and the antioxidant and antibacterial effects of each sample were determined.The pharmacodynamic indexes and common peak areas were analyzed by partial least square regression,grey correlation and correlation analysis using SPSS software.Results The correlation degree analysis showed that peaks 3 and 12 were strongly correlated with antioxidant and antibacterial.The regression coefficient of partial least square regression showed that peaks 2 and 3 were strongly correlated with antioxidant effect,and the components of peaks 2,3,5,7,9,13,17 and 18 were strongly correlated with antibacterial effect.The bivariate correlation analysis showed that peak 3 was significantly correlated with antioxidant effect,and peaks 4,11 and 12 were significantly correlated with antibacterial effect.According to the reference substance,peak 3 was chlorogenic acid,peak 7 was caffeic acid,peak 11 was isochlorogenic acid B,peak 12 was isochlorogenic acid A,peak 17 was lonicera macranthoides saponin A,peak 18 was dipsacus saponin B,and the components of peaks 2,5,9 and 13 needed to be further confirmed.Conclusion The"spectrum-effect"and"dose-effect"relationships between antioxidant and antibacterial of Flos lonicerae are analyzed by various analytical methods.The results are consistent and mutually confirmed.It is predicted that organic acids are the quality markers of antioxidant,saponins and organic acids are the quality markers of antibacterial.

关 键 词：灰毡毛忍冬 抗氧化 抑菌 谱-效关系 量-效关系 质量标志物 

分 类 号：R284.1[医药卫生—中药学]