藏药俄色对自发性2型糖尿病KK-Ay小鼠胰岛素抵抗作用  

Effect of Tibetan medicine E-se on insulin resistance in KK-Ay mice with spontaneous type 2 diabetes

作　　者：华桦[1] 刘芳[2] 刘俐 罗园 曾安琪 戴瑛[1] 尹竹君 赵军宁[1] HUA Hua;LIU Fang;LIU Li;LUO Yuan;ZENG An-qi;DAI Ying;YIN Zhu-jun;ZHAO Jun-ning(Sichuan Academy of Chinese Medicine Sciences,Sichuan Institute for Translational Chinese Medicine,Biological Assay Key Laboratory of State Administration of Traditional Chinese Medicine for Traditional Chinese Medicine Quality,Engineering Research Center for Formation Principle and Quality Evaluation of Genuine Medicinal MateMaterials in Sichuan Province,Sichuan Engineering Technology Research Center of Genuine Regional Drug,Translational Chinese Medicine Key Laboratory of Sichuan Province;West China School of Pharmacy,Sichuan University;Chengdu Integrated TCM&Western Medicine Hospital,Chengdu 610041,China)

机构地区：[1]四川省中医药科学院、四川省中医药转化医学中心国家中医药管理局中药质量生物评价重点研究室、四川省道地药材形成原理与品质评价工程研究中心四川省道地药材系统开发工程技术研究中心中医药转化医学四川省重点实验室 [2]四川大学华西药学院 [3]成都市中西医结合医院,四川成都610041

出　　处：《中国药理学通报》2022年第8期1252-1259,共8页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年科学基金项目(No.81703820);四川省科技厅重点研发项目(No.2018SZ0056)。

摘　　要：目的评价俄色提取物对自发性2型糖尿病KK-Ay小鼠胰岛素抵抗的改善作用及其机制。方法10只C57/6J小鼠为正常对照组,KK-Ay模型小鼠50只,随机分为5组(n=10):①模型组;②阳性组:罗格列酮(2.67 mg·kg^(-1));③俄色低剂量组:0.75 g生药·kg^(-1);④俄色中剂量组:1.50 g生药·kg^(-1);⑤俄色高剂量组:3.00 g生药·kg^(-1);每周测体质量和空腹血糖;d 32测胰岛素耐量;d 35末次给药后测胰岛素,计算胰岛素抵抗指数和敏感指数;胰腺HE;TUNEL染色检测胰岛细胞凋亡;免疫组化测胰高血糖素样肽-1(GLP-1)。结果与模型组比较,俄色组小鼠体质量、空腹血糖值、血清胰岛素浓度、胰岛素抵抗指数降低;胰岛素敏感指数升高;胰腺组织病变分级评分和胰岛细胞凋亡百分比降低;胰腺组织GLP-1蛋白含量增加。结论俄色提取物可通过降低血清胰岛素水平、减少胰岛细胞凋亡和增加机体对胰岛素的敏感性,从而改善胰岛素抵抗。Aim To evaluate the effect of E-se extract on insulin resistance in KK-Ay mice with spontaneous type 2 diabetes and explore its mechanism.Methods Ten C57/6J mice were assigned to a normal control group.Fifty KK-Ay model mice were randomly divided into model group,positive control group(rosiglitazone,2.67 mg·kg^(-1)),and low-(0.75 g·kg^(-1)),medium-(1.50 g·kg^(-1)),and high-dose(3.00 g·kg^(-1))E-se groups,with 10 mice in each group.All mice were measured for body weight and fasting blood glucose weekly,insulin tolerance on the 32nd day,and insulin after the last administration on the 35th day,and the insulin resistance/sensitivity indexes were calculated.The pancreas was stained by hematoxylin-eosin(HE).Islet cell apoptosis was detected by TUNEL staining.Glucagon-like peptide-1(GLP-1)was detected by immunohistochemistry.Results Compared with the model group,the E-se groups showed reduced body weight,fasting blood glucose,serum insulin concentration,and insulin resistance index,elevated insulin sensitivity index,decreased lesion grading score of pancreatic tissues and apoptosis percentage of islet cells,and increased content of GLP-1 protein in pancreatic tissues.Conclusions E-se extract can improve insulin resistance by reducing serum insulin level,inhibiting islet cell apoptosis,and increasing the sensitivity of the body to insulin.

关 键 词：俄色 变叶海棠 糖尿病 KK-AY小鼠 胰岛素抵抗 作用机制 

分 类 号：R-332[医药卫生] R285.5R329.25R458.5R587.1