谷氨酸棒杆菌生产莽草酸的代谢工程研究进展  

Research Progress in Metabolic Engineering of Shikimic Acid Production by Corynebacterium Glutamicum

作　　者：沈跃峰 尹良鸿 钱永常[1] SHEN Yue-feng;YIN Liang-hong;QIAN Yong-chang(College of Food and Health,Zhejiang A&F University,Hangzhou 311300,China)

机构地区：[1]浙江农林大学食品与健康学院,浙江杭州311300

出　　处：《药物生物技术》2022年第3期300-304,共5页Pharmaceutical Biotechnology

基　　金：国家自然科学基金(No.31600070);浙江农林大学个人科研发展基金(No.2015FR015)。

摘　　要：莽草酸是从中药八角茴香中提取的一种单体化合物,是工业生产吲哚衍生物、手性药物中的关键原料,是抗流感药物“达菲”的重要前体。莽草酸可以通过植物提取法、化学合成法及微生物发酵法生产。其中微生物发酵法具有原料廉价易得、环境友好、可持续性等优点。文章分析了谷氨酸棒杆菌莽草酸合成代谢途径及代谢调控,综述了近年来关于莽草酸代谢工程改造策略的研究进展,并展望了莽草酸生物合成的未来发展趋势。Shikimic acid was a monomer compound extracted from star anise,which was a key synthetic raw material in industrial production and an important precursor of anti influenza drug “Tamiflu”. Shikimic acid was produced by plant extraction,chemical synthesis,and microbial fermentation. Among them,microbial fermentation had the advantages of cheap and easy to obtain raw materials,environmental friendliness and sustainability. The biosynthesis pathway and metabolic regulation of shikimic acid in Corynebacterium glutamicum were systematically analyzed,the research progress of shikimic acid metabolism engineering modification strategy in recent years were summarized in this review,the future development trend of shikimic acid biosynthesis were prospected.

关 键 词：谷氨酸棒杆菌 莽草酸 莽草酸途径 代谢工程 微生物发酵 合成生物学 

分 类 号：Q815[生物学—生物工程]