乳腺肿瘤细胞体外分离培养方法的研究进展  

Research Progress in Isolation and Culture of Breast Tumor Cells in Vitro

作　　者：邱敏 陈柳[2] 代解杰 QIU Min;CHEN Liu;DAJ Jiejie(Kunming Medical University,Kunming 650500,China;Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming 650118,China)

机构地区：[1]昆明医科大学,昆明650500 [2]中国医学科学院北京协和医学院医学生物学研究所,昆明650118

出　　处：《医学综述》2022年第14期2790-2794,2800,共6页Medical Recapitulate

基　　金：昆明市科技计划项目(2019-1-R-24483)。

摘　　要：乳腺癌是女性常见的恶性肿瘤之一,其发病率逐年升高。建立乳腺癌细胞系或细胞株,对于开展乳腺癌病因、发病机制、防治及抗癌药物筛选等方面的研究均具有重要意义。目前乳腺癌细胞的分离培养方法主要包括酶消化分离法、机械分离法、组织块培养法、三维细胞培养技术以及条件重编程细胞培养技术等,这些分离培养方法在生物学特性与应用方面各具特色。因此,进一步归纳总结乳腺癌细胞的分离培养方法可以为未来乳腺癌的相关研究提供理论基础。Breast cancer is one of the common malignant tumors in women,and its incidence is increasing year by year.The establishment of breast cancer cell lines or cell strains is of great significance for the study of breast cancer etiology,pathogenesis,prevention and treatment and anti-cancer drug screening.At present,the methods of isolation and culture of breast cancer cells mainly include enzyme digestion and separation,mechanical separation,tissue culture,three-dimensional cell culture and conditional reprogramming cell culture.These methods have their own characteristics in biological characteristics and application.Therefore,further summarizing the isolation and culture methods of breast cancer cells can provide a theoretical basis for the future research on breast cancer.

关 键 词：乳腺肿瘤 分离培养 三维细胞培养技术 条件性重编程细胞技术 

分 类 号：R733[医药卫生—肿瘤]