Chlorogenic acid alleviates hypoxic-ischemic brain injury in neonatal mice  

作　　者：Lu-Yao Li Qi Wang Lu Deng Zhen Lin Jing-Jing Lin Xin-Ye Wang Tian-Yang Shen Yi-Hui Zheng Wei Lin Pei-Jun Li Xiao-Qin Fu Zhen-Lang Lin 

机构地区：[1]Department of Pharmacy,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,Zhejiang Province,China [2]Department of Neonatology,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,Zhejiang Province,China [3]Department of Geriatrics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,Zhejiang Province,China [4]Department of Orthopedics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,Zhejiang Province,China [5]Department of Neurology,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,Zhejiang Province,China

出　　处：《Neural Regeneration Research》2023年第3期568-576,共9页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81971425;the Natural Science Foundation of Zhejiang Province,No.LY20H040002(both to XQF).

摘　　要：Recent studies have shown that chlorogenic acid(CGA),which is present in coffee,has protective effects on the nervous system.However,its role in neonatal hypoxic-ischemic brain injury remains unclear.In this study,we established a newborn mouse model of hypoxic-ischemic brain injury using a modified Rice-Vannucci method and performed intraperitoneal injection of CGA.We found that CGA intervention effectively reduced the volume of cerebral infarct,alleviated cerebral edema,restored brain tissue structure after injury,and promoted axon growth in injured brain tissue.Moreover,CGA pretreatment alleviated oxygen-glucose deprivation damage of primary neurons and promoted neuron survival.In addition,changes in ferroptosis-related proteins caused by hypoxic-ischemic brain injury were partially reversed by CGA.Furthermore,CGA intervention upregulated the expression of the key ferroptosis factor glutathione peroxidase 4 and its upstream glutamate/cystine antiporter related factors SLC7A11 and SLC3A2.In summary,our findings reveal that CGA alleviates hypoxic-ischemic brain injury in neonatal mice by reducing ferroptosis,providing new ideas for the treatment of neonatal hypoxic-ischemic brain injury.

关 键 词：chlorogenic acid ferroptosis glutathione peroxidase 4 lipid peroxidation neonatal hypoxic-ischemic brain injury NEURONS NEUROPROTECTION oxidative stress oxygen-glucose deprivation system Xc^(-) 

分 类 号：R651.15[医药卫生—外科学]