Hypoxic pre-conditioned adipose-derived stem/progenitor cells embedded in fibrin conduits promote peripheral nerve regeneration in a sciatic nerve graft model  

作　　者：Julius M.Mayer Christian Krug Maximilian M.Saller Annette Feuchtinger Riccardo E.Giunta Elias Volkmer Thomas Holzbach 

机构地区：[1]Department of Hand-and Plastic Surgery,Spital Thurgau,Frauenfeld,Switzerland [2]Musculoskeletal University Center Munich(MUM),Department of Orthopedics and Trauma Surgery,Ludwig-Maximilians-University(LMU),Planegg-Martinsried,Germany [3]Research Unit Analytical Pathology,Munich,Helmholtz Zentrum München-German Research Center for Environmental Health(GmbH),Neuherberg,Germany [4]Division of Hand-,Plastic-and Aesthetic Surgery,Ludwig-Maximilians-University(LMU),Munich,Germany [5]Division of Hand Surgery,Helios Klinikum München West,Munich,Germany

出　　处：《Neural Regeneration Research》2023年第3期652-656,共5页中国神经再生研究（英文版）

基　　金：support by the Faculty of Medicine,Ludwig-Maximilians-University(FöFoLe,Project 843 and 955,to TH and MMS).

摘　　要：Recent results emphasize the supportive effects of adipose-derived multipotent stem/progenitor cells(ADSPCs)in peripheral nerve recovery.Cultivation under hypoxia is considered to enhance the release of the regenerative potential of ADSPCs.This study aimed to examine whether peripheral nerve regeneration in a rat model of autologous sciatic nerve graft benefits from an additional custom-made fibrin conduit seeded with hypoxic pre-conditioned(2%oxygen for 72 hours)autologous ADSPCs(n=9).This treatment mode was compared with three others:fibrin conduit seeded with ADSPCs cultivated under normoxic conditions(n=9);non-cell-carrying conduit(n=9);and nerve autograft only(n=9).A 16-week follow-up included functional testing(sciatic functional index and static sciatic index)as well as postmortem muscle mass analyses and morphometric nerve evaluations(histology,g-ratio,axon density,and diameter).At 8 weeks,the hypoxic pre-conditioned group achieved significantly higher sciatic functional index/static sciatic index scores than the other three groups,indicating faster functional regeneration.Furthermore,histologic evaluation showed significantly increased axon outgrowth/branching,axon density,remyelination,and a reduced relative connective tissue area.Hypoxic pre-conditioned ADSPCs seeded in fibrin conduits are a promising adjunct to current nerve autografts.Further studies are needed to understand the underlying cellular mechanism and to investigate a potential application in clinical practice.

关 键 词：adipose-derived progenitor cells adipose-derived multipotent stem/progenitor cell autologous nerve graft fibrin conduit hypoxia hypoxic pre-conditioning nerve defect nerve tissue engineering peripheral nerve regeneration regenerative medicine 

分 类 号：R741[医药卫生—神经病学与精神病学]