Therapeutic effects of the extract of Sancao Formula, a Chinese herbal compound, on imiquimod-induced psoriasis via cysteine-rich protein 61  

作　　者：Wan-jun Guo Yi Wang Yu Deng Lin-yan Cheng Xin Liu Ruo-fan Xi Sheng-jie Zhu Xin-yi Feng Liang Hua Kan Ze Jian-yong Zhu Dong-jie Guo Fu-lun Li 

机构地区：[1]Department of Dermatology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China [2]School of Medicine,Chengdu University,Chengdu 610106,Sichuan Province,China [3]Institute of Cancer Biology and Drug Discovery,Chengdu University,Chengdu 610106,Sichuan Province,China [4]Clinical Laboratory Medicine Center,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China

出　　处：《Journal of Integrative Medicine》2022年第4期376-384,共9页结合医学学报（英文版）

基　　金：supported by National Natural Science Foundation of China (No. 81803982, 82004366, and 82074428);Clinical Research Plan of Shanghai Shenkang Hospital Development Center(No. SHDC2020CR4041);Shanghai Municipal Commission of Science and Technology (No. 20XD1423600, 20DZ2301900);Sailing Program of Shanghai Rising-Star Program (No. 22YF1449700);the Project of Shanghai University of Traditional Chinese Medicine(No. Y2020064, Y2021019);Shanghai Municipal Commission of Economy and Information Technology,Shanghai Artificial Intelligence Innovation and Development Project-Intelligent Dermatology Clinic Based on Modern TCM Diagnostic Technology (No. 2020-RGZN-02038)。

摘　　要：Objective: Psoriasis is a common chronic inflammatory skin disease that is prone to recurrence, and the proinflammatory factor, cysteine-rich protein 61(Cyr61), is important in its pathophysiology. Long-term clinical practice has shown that Sancao Formula(SC), a Chinese herbal compound, is effective in the treatment of psoriasis, but the precise mechanism remains unknown. In this study, we investigate the mechanism by which SC extract alleviates imiquimod(IMQ)-induced psoriasis.Methods: The expression of Cyr61 in psoriatic lesions and normal healthy skin was detected using immunohistochemical analysis to investigate the biological role of Cyr61 in models of psoriatic inflammation. A psoriatic mouse model was established by topical application of IMQ, and the effect of topical application of SC extract was evaluated using the psoriasis area and severity index(PASI) score,hematoxylin-eosin staining, and histopathological features of the skin. Next, a HaCaT cell inflammation model was established using interferon-γ(IFN-γ), and the effect of SC extract on the mRNA and protein levels of Cyr61 and intercellular cell adhesion molecule-1(ICAM-1) was confirmed using Western blot and quantitative real-time polymerase chain reaction analyses.Results: Immunohistochemical staining showed that the expression of Cyr61 in psoriatic lesions was higher than that in normal skin samples(78.26% vs 41.18%, P < 0.05), and the number of Cyr61-positive cells in psoriatic lesions was also significantly higher than in normal skin(18.66 ± 2.51 vs4.33 ± 1.52, P < 0.05). Treatment in mice with IMQ-induced psoriasis showed that SC extract could significantly improve the inflammatory phenotype, PASI score(10.875 ± 0.744 vs 3.875 ± 0.582, P < 0.05),and pathological features compared with those in IMQ model group;SC treatment was also associated with decreased levels of Cyr61 and ICAM-1. In the IFN-γ-induced inflammatory cell model, the mRNA and protein levels of Cyr61 and ICAM-1 were upregulated, while the SC extract downregulated the lev

关 键 词：Traditional Chinese medicine Sancao Formula PSORIASIS Plant extracts Cysteine-rich protein 61 Intercellular cell adhesion molecule-1 

分 类 号：R275.9[医药卫生—中西医结合]