补肾通络方对去势大鼠骨质疏松症模型子宫内膜和骨量流失的影响  

Effect of Bushen Tongluo Recipe(补肾通络方)on Endometrium and Bone Lost in Ovariectomized Rats with Osteoporosis

作　　者：凌家艳[1] 刘庆[2] 马威[1] 薛莎[1] LING Jiayan;LIU Qing;MA Wei;XUE Sha(Wuhan NO.1 Hospital,Wuhan Hubei 430030,China;Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan Hubei 430061,China)

机构地区：[1]武汉市第一医院,湖北武汉430030 [2]湖北省中医院,湖北武汉430061

出　　处：《中医药导报》2022年第6期20-23,34,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：武汉市卫生计生科研基金项目(WZ16A05);武汉市医学骨干人才培养工程(武卫生计生〔2016〕59号)。

摘　　要：目的:探讨补肾通络方对去势骨质疏松症模型大鼠子宫内膜和骨量流失的影响。方法:40只SPF级雌性SD大鼠随机分成假手术组(8只)和造模组(32只)。假手术组大鼠切除卵巢周围少量脂肪,造模组大鼠行双侧卵巢切除术进行造模,造模全部成功后随机分为模型组、雌激素组、补肾通络方高剂量组、补肾通络方低剂量组,每组8只。各组大鼠分别给予生理盐水、生理盐水、戊酸雌二醇、高剂量补肾通络方、低剂量补肾通络方灌胃,3个月后检测血清雌二醇(E_(2))、卵泡刺激素(FSH)、黄体生成素(LH)水平。HE染色观察子宫内膜变化,免疫组化检测子宫内膜雌激素受体(ERα)、β-连环蛋白(β-catenin)表达。Micro-CT检测大鼠第四腰椎骨密度及骨微结构的变化。结果:与假手术组比较,模型组大鼠血清E_(2)含量明显降低(P<0.05);FSH、LH水平均明显升高(P<0.05),第四腰椎骨密度明显降低(P<0.05),骨微结构破坏明显;大鼠子宫内膜上皮及腺上皮ERα和β-catenin表达水平均明显降低(P<0.05)。与模型组比较,补肾通络方低、高剂量组和戊酸雌二醇组大鼠血清E_(2)含量明显升高(P<0.05),FSH、LH水平均明显降低(P<0.05),第四腰椎骨密度均明显升高(P<0.05);骨微结构明显改善;大鼠子宫内膜上皮及腺上皮ERα和β-catenin表达水平均明显升高(P<0.05)。补肾通络方低、高剂量组各指标与戊酸雌二醇组比较,差异均无统计学意义(P>0.05)。结论:补肾通络方能改善去势骨质疏松症模型大鼠性激素水平及骨密度;补肾通络方可能通过上调去卵巢大鼠子宫内膜ERα表达,调控Wnt/β-catenin信号通路,从而发挥防治绝经后骨质疏松症的作用。Objective:To investigate the effect of Bushen Tongluo Recipe on bone lost and endometrium in ovariectomized rats.Methods:40 SPF female SD rats were randomly divided into sham operation group(8rats)and model group(32rats).The rats in the sham operation group removed a small amount of fat around the ovaries,and the rats in the model group underwent bilateral oophorectomy for modeling.After successful modeling,they were randomly divided into model group,estrogen group,Bushen Tongluo Recipe high and low dose groups,with 8 rats in each group.Each group were given normal saline,normal saline,estradiol valerate,high and low dose Bushen Tongluo Recipe by gavage respectively.After 3 months,serum estradiol(E_(2)),follicle stimulating hormone(FSH)and luteinizing hormone(LH)levels were detected.Endometrium changes was observed by HE staining,and the expressions of ERα and β-catenin were detected by immunohistochemistry.Changes of bone mineral density and bone microstructure of the fourth lumbar spine were detected by Micro-CT.Results:Compared with the sham operation group,the serum E_(2) content of the model group was significantly lower(P<0.05).The levels of FSH and LH increased significantly(P<0.05).The bone mineral density of the fourth lumbar vertebra decreased significantly(P<0.05),and the bone microstructure was damaged significantly.The expression levels of Erα and β-catenin in endometrial and glandular epithelium of rats were significantly decreased(P<0.05).Compared with the model group,the serum E_(2) content of high and low dose Bushen Tongluo Recipe groups increased significantly(P<0.05),and the levels of FSH and LH were decreased significantly(P<0.05).The bone mineral density of the fourth lumbar vertebra increased significantly(P<0.05).Bone microstructure was significantly improved.The expressions level of ERα and β-catenin in endometrial and glandular epithelium of rats were both decreased significantly(P<0.05).There was no significant difference between the low and high dose groups of Bushen Tongluo Reci

关 键 词：骨质疏松症 绝经后骨质疏松症 去势大鼠 补肾通络方 雌激素 ERΑ Β-CATENIN 

分 类 号：R285.5[医药卫生—中药学]