八种外生菌根菌与黑松菌根合成及其特征  

Mycorrhiza Synthesis of Pinus thunbergii with Eight Ectomycorrhizal Fungi Species

作　　者：黄兰兰 万山平[1] 汪延良 于莺曼 时晓菲[2] 于富强[2] HUANG Lanlan;WAN Shanping;WANG Yanliang;YU Yingman;SHI Xiaofei;YU Fuqiang(College of Resources and Environment,Yunnan Agricultural University,Kunming 650204,Yunnan,China;The Germplasm Bank of Wild Species,Yunnan Key Laboratory for Fungal Diversity and Green Development,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,Yunnan,China;School of Life Science,South China Normal University,Guangzhou 510631,Guangdong,China)

机构地区：[1]云南农业大学资源与环境学院,云南昆明650201 [2]中国科学院昆明植物研究所,中国西南野生生物种质资源库,云南省真菌多样性与绿色发展重点实验室,云南昆明650204 [3]华南师范大学生命科学学院,广东广州510631

出　　处：《食用菌学报》2022年第4期98-106,共9页Acta Edulis Fungi

基　　金：云南省重大科技专项计划(202102AE090034);云南省基础研究计划项目(202201 AT 070268);国家重大研发计划项目(2021YFD1600404)。

摘　　要：为探讨不同外生菌根真菌与黑松(Pinus thunbergii)的共生特性,选用黑孢块菌(Tuber melanosporum)、丽江块菌(T.lijiangense)、假喜马拉雅块菌(T.pseudohimalayense)、台湾块菌(T.formosanum)、波氏块菌(T.borchii)、印度块菌(T.indicum)、松毛蛋须腹菌(Rizopogen songmaodan)和彩色豆马勃(Pisolithus tinctorius)8种外生菌根真菌,采用孢子接种与黑松进行菌根合成,对接种后菌根的侵染情况、形态和解剖特征进行分析。结果表明:除黑孢块菌、印度块菌和彩色豆马勃外,其余5种真菌均与黑松形成菌根。丽江块菌、假喜马拉雅块菌、台湾块菌和波氏块菌与黑松形成菌根的形态较为相似,呈二叉状或珊瑚状,颜色呈黄棕色至深棕色,外菌套呈典型的迷宫状;丽江块菌和波氏块菌与黑松形成菌根的外延菌丝呈刚毛状,假喜马拉雅块菌和台湾块菌与黑松形成菌根的外延菌丝呈卷曲毛发状。松毛蛋须腹菌与黑松形成的菌根呈棒状,表面包裹一层白色的外延菌丝。The symbiotic characteristics of different ectomycorrhizal fungi and Pinus thunbergii were explored.Axenically germinated seedlings of P.thunbergii in greenhouse were inoculated with spore suspensions of eight ectomycorrhizal fungi(EMF)species,including Tuber melanosporum,T.lijiangense,T.pseudohimalayense,T.formosanum,T.borchii,T.indicum,Rhizopogon songmaodan and Pisolithus tinctorius,respectively.The obtained mycorrhizae were analyzed for morphological and anatomical characteristics.Six months after inoculation,P.thunbergii successfully formed mycorrhizal roots with T.lijiangense,T.pseudohimalayense,T.formosanum,T.borchii and R.songmaodan.The mycorrhizae of P.thunbergii with T.lijiangense,T.pseudohimalayense,T.formosanum,T.borchii looked similar in morphology,showing a typical dichotomous or irregularly pinnate appearance,yellow-brownish to dark-brownish color,and a typical maze-like outer mantle.The mycorrhizae of P.thunbergii with T.lijiangense and T.borchii had spiky and hyaline emanating cystidiae,while the mycorrhizae of P.thunbergii with T.pseudohimalayense and T.formosanum produced long hyaline emanating hyphae with right-angle ramifications.The mycorrhizal roots formed by P.thunbergii and R.songmaodan were rod-shaped and covered with a layer of white emanating hyphae.

关 键 词：块菌 松毛蛋须腹菌 彩色豆马勃 孢子接种 污染 

分 类 号：S646[农业科学—蔬菜学]