解吸电喷雾电离质谱成像技术及其在文件/文物检验中的应用  

DESI-MSI and Its Application into Examination of Document/Cultural Relics

作　　者：李志豪 王蔚昕[1] 石高军[1] 梁鲁宁[1] 光晓俐 齐凤亮[1] 廉哲[1] 张健 邹积鑫[1] LI Zhihao;WANG Weixin;SHI Gaojun;LIANG Luning;GUANG Xiaoli;QI Fengliang;LIAN Zhe;ZHANG Jian;ZOU Jixin(Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)

机构地区：[1]公安部物证鉴定中心,北京100038

出　　处：《刑事技术》2022年第4期342-346,共5页Forensic Science and Technology

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2022JB013)。

摘　　要：质谱成像技术是一种通过原位分析获得被分析物空间分布信息的新型成像技术。解吸电喷雾电离质谱成像技术因其常压敞开式操作、前处理简便、对样品破坏少、高通量等优势成为近年来质谱成像领域的焦点。本文对该技术自诞生以来在文件/文物检验领域的应用及相关文献进行综述,归纳出该技术的原理及其在文件/文物检验研究中的主要应用成果及存在问题,并对其应用前景进行了展望,以期为文件检验工作者提供参考。Mass spectrometry imaging is a new approach of imaging technology,capable of providing rich in situ spatial distribution information about the analyte.Desorption electrospray ionization(DESI)-mass spectrometry imaging(MSI)has in recent years been focused owing to its merits of ambient operation,simple pre-processing,less damage to the treated sample and high throughput.DESI plays its role through the solvent(usually methanol or isopropyl alcohol)being sprayed onto the surface of sample via capillary atomizing at open natural environment so that the desorption and ionization could be carried out meanwhile.During the process,the sampling ions would pass by droplet through the ion transfer tube to enter the mass spectrometer for detection.However,if the droplet fl ow is sprayed speedily,smearing phenomena would appear to result in damaging the tested document and analyte(e.g.,ink).The another infl uential factor is the distance between the mass spectrometer’s sampling inlet and the analyte,also able to affect the sampling integrity.DESI-MSI has been widely used with environmental/food testing,criminal forensic detection and other fi elds due to its simple sample preparation and experimental operation.For example,document/cultural relic inspection is usually related to testing relevant polar compounds and art masterpiece of high price value,DESI-MSI is thus an appropriate choice to undertake the inspection/examination.When DESI-MSI is used to analyze document materials,each sampling micro-area could be extracted of the charge-to-massratio images up to more than 1000 varieties at the same time,therewith bringing forth a large amount of data.Chemometric methods,e.g.,principal component analysis and partial least squares-discriminant analysis,would be available to modularly group and analyze the data of mass spectrometry to identify differences and/or conclude results.Accordingly,species discrimination and source tracking could be conducted with the identifi ed differences and conlcuded results.At present,DESIMSI is s

关 键 词：文件检验 质谱成像 解吸电喷雾电离 墨迹 

分 类 号：DF794.2[政治法律—诉讼法学]