大熊猫粪便中mtDNA拷贝数与月龄的关系  

The Relationship Between mtDNA Copy Number and Age in Giant Panda Feces

作　　者：任晓彤 周永恒 李天峰 李仁贵 马跃[1,4] 李德生 黄炎 徐艳春 REN Xiaotong;ZHOU Yongheng;LI Tianfeng;LI Rengui;MA Yue;LI Desheng;HUANG Yan;XU Yanchun(College of Wildlife and Protected Area,Northeast Forestry University,Harbin,150040,China;China Conservation and Research Center for Giant Panda,Dujiangyan,611830,China;Key Laboratory of National Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park,Dujiangyan,611830,China;National Forestry and Grassland Administration Detecting Center of Wildlife,Harbin,150040,China;National Forestry and Grassland Administration Research Center of Technology for Wildlife Conservation and Utilization,Harbin,150040,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院,哈尔滨150040 [2]中国大熊猫保护研究中心,都江堰611830 [3]大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室,都江堰611830 [4]国家林业和草原局野生动植物检测中心,哈尔滨150040 [5]国家林业和草原局野生动物保护与利用工程技术研究中心,哈尔滨150040

出　　处：《野生动物学报》2022年第3期676-684,共9页CHINESE JOURNAL OF WILDLIFE

基　　金：国家自然科学基金面上项目(32170517);大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室开放课题(KLSFGAGP2020.017)。

摘　　要：动物在生长发育不同阶段组织器官的代谢水平有所差异,线粒体DNA(mtDNA)的拷贝数在一定程度上反映了机体的生理状态和代谢强度。以64只大熊猫(Ailuropoda melanoleuca)粪便DNA为材料,采用实时荧光定量PCR方法,验证了肠道上皮组织中平均每个细胞mtDNA的拷贝数(N_(mpc))与月龄之间具有显著相关性(R^(2)=0.129,P=0.004),且呈现出随着月龄的增长而下降的趋势。幼龄个体的N_(mpc)远高于中、老龄个体,且个体差异很大。此外,性别对N_(mpc)也具有明显的影响,同一生长阶段的雄性比雌性更高。这些发现为进一步探究肠道上皮细胞mtDNA拷贝数与生长发育和衰老之间的关系,以及利用其作为指标来评估机体的生理状况提供了依据。Metabolic rate varies with age in animal tissues and organs.The copy number of mtDNA reflects the physiological state and metabolic level of a tissue to a certain extent.Therefore,the copy number of mtDNA might associate with age.In this study,we quantified the average copy number of mtDNA per cell(N_(mpc))in fecal DNA representing shed epithelial cells of hind gut of 64 giant pandas(Ailuropoda melanoleuca)by using quantitative real-time PCR approach.The results showed a significant,but weak negative correlation between N mpc and age(R^(2)=0.129,P=0.004).N_(mpc) of young pandas varied greatly,but was significantly higher than that of middle-aged and old individuals.Meanwhile,it was found that gender had influence on copy number of mtDNA.N_(mpc) of males was greater than that of females at all age classes.These findings provided a basis for further probes into the relationship of N_(mpc) of epithelial cells and growth,development and aging of gut,and suggested new perspective for the use of feces to infer the physiological state of animals.

关 键 词：大熊猫 粪便DNA mtDNA拷贝数 月龄 

分 类 号：Q953[生物学—动物学]