小熊猫犬瘟热病毒分离鉴定及其H基因序列分析  

Isolation and Identification of Canine Distemper Virus in Red Panda and Sequence Analysis of the H Genes

作　　者：陈诗涵 黄紫贝 管飘萍 郭鑫 王海燕[3] 金文杰[1,4] 刘文博 CHEN Shihan;HUANG Zibei;GUAN Piaoping;Guo Xin;WANG Haiyan;JIN Wenjie;LIU Wenbo(College of Veterinary Medicine,Yangzhou University,Yangzhou,225009,China;Collaborative Innovation Center for Prevention and Control of Important Animal Diseases and Zoonosis in Jiangsu Province,Yangzhou,225009,China;Jiangsu Vocational College of Agriculture and Animal Husbandry,Taizhou,225300,China;Animal Disease Testing and Technical Service Center of Yangzhou University,Yangzhou,225009,China)

机构地区：[1]扬州大学兽医学院传染病学教研室,扬州225009 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心,扬州225009 [3]江苏农牧科技职业学院,泰州225300 [4]扬州大学动物疾病检测与技术服务中心,扬州225009

出　　处：《野生动物学报》2022年第3期685-691,共7页CHINESE JOURNAL OF WILDLIFE

基　　金：江苏省优势学科第三期(2018)资助项目。

摘　　要：2019年10月21日—12月中旬,江苏某动物园小熊猫(Ailurus fulgens)出现疑似犬瘟热病毒(Canine distemper virus,CDV)感染,用犬瘟热胶体金试纸条检测,结果为弱阳性,共12只小熊猫死亡。对病死小熊猫剖检,可见明显的肺充血、出血,肝肿大,心内膜出血,胃肠黏膜出血。采集2只发病小熊猫尾静脉血送检,RT-PCR检测犬瘟热核酸为阳性,用Vero-CD150细胞对样品进行病毒分离,在Vero细胞上连续传代,通过RT-PCR检测,病毒核酸仍然为阳性,最终分离到2株犬瘟热病毒,分别命名为SZ2019CDV1H和SZ2019CDV2H。扩增H基因并测序,与GenBank已公布的基因序列进行同源性比较和遗传发生分析,SZ2019CDV1H株与SZ2019CDV2H株的基因同源性为97.2%。在遗传发生关系树上,SZ2019CDV1H株为Africa毒株,SZ2019CDV2H株为European wildlife毒株,分属于不同的遗传分支。From 21 to mid-December,2019,some red pandas(Ailurus fulgens)suspected to be infected with canine distemper virus(CDV)in a zoo park in Jiangsu,and tested weakly positive using canine distemper immunocolloidal gold test strips.12 individuals were dead because of that.The gross lesions of the dead red panda appeared obvious pulmonary congestion,hepatomegaly,hemorrhage of lungs,endocardia and gastrointestinal mucosal.Blood samples from tail vein of two sick red pandas were tested and a RT-PCR test for canine distemper virus specific nucleic acid showed positive.Themonolayer of Vero-CD150 cells were inoculated to isolate the virus fromblood samples,and passaged serially on the cells,verified by RT-PCR,the test results of viral nucleic acid were still positive,and finally two strains of canine distemper were isolated.The viruses were referred to as SZ2019CDV1H and SZ2019CDV2H respectively.The H gene was amplified and sequenced,and the homology was compared with the related sequences in GenBank,then the variation of the H genes were determined.The results showed although two isolates have high homology(97.2%),SZ2019CDV1H was belong to an African canine distemper strain,and SZ2019CDV2H was belong to an European wild animal canine distemper strain.The two isolates were apart from phylogenetic relationship.

关 键 词：小熊猫 犬瘟热病毒 分离鉴定 H基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]