桑葚花色苷提取物对乙醇诱导的小鼠肝损伤和死亡率的影响  

Effects of Cyanidin-3-O-Glucoside Extracted from Mulberry on Ethanol-Induced Liver Injury and Mortality of Mice

作　　者：李文丽 周羽佳 杨丽丽[1] LI Wen-li;ZHOU Yu-jia;YANG Li-li(Department of Nutrition,School of Public Health,Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学公共卫生学院营养与食品卫生学系,广州510080

出　　处：《中国食物与营养》2022年第7期34-40,共7页Food and Nutrition in China

基　　金：国家自然科学基金(项目编号:81872613)。

摘　　要：目的:探讨桑葚花色苷提取物矢车菊素-3-O-葡萄糖苷(Cy-3-G)对乙醇诱导的小鼠肝损伤和死亡率的影响。方法:将8w龄雄性C57BL/6J小鼠随机分为对照组、酒精液体饲料喂养组以及酒精液体饲料喂养加Cy-3-G干预组。对照组喂食Lieber-DeCarli对照液体饲料,后两组喂食Lieber-DeCarli酒精液体饲料构建小鼠酒精性肝损伤模型,Cy-3-G以灌胃方式给予。观察肝脏组织病理学改变;检测肝脏总胆固醇(TC),血清天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT),及血清内毒素(LPS)等生化指标水平;检测肝脏促炎细胞因子白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和巨噬细胞趋化蛋白-1(MCP-1)水平;记录小鼠死亡情况。结果:短期乙醇喂养使小鼠肝脏出现明显脂肪变性,肝脏TC,血清ALT、AST水平升高,而Cy-3-G干预缓解了乙醇诱导的小鼠肝脏脂肪变性,降低了小鼠肝脏TC,血清ALT、AST水平。与酒精组相比,Cy-3-G补充可以降低血清LPS水平,并进一步改善乙醇引起的肝脏炎症,降低IL-1β、TNF-α和MCP-1水平。长期过量乙醇摄入导致小鼠死亡率增加,而Cy-3-G干预明显降低小鼠死亡率。结论:桑葚花色苷提取物Cy-3-G可以改善乙醇诱导的小鼠肝损伤,其保护机制可能是通过抑制LPS释放从而抑制肝脏炎症反应实现的。此外Cy-3-G还可以有效预防长期过量乙醇摄入导致的小鼠死亡,保护小鼠健康,提示Cy-3-G有望成为防治酒精性健康损害的有效措施。Objective To investigate the effects of Cyanidin-3-O-Glucoside(Cy-3-G)extracted from mulberry on ethanol-induced liver injury and mortality of mice.Method Male C57BL/6J mice(8 weeks old)were randomly divided into control group,ethanol group and ethanol+Cy-3-G group.The control group was fed Lieber-DeCarli control liquid diet and the latter two groups were fed Lieber-DeCarli thanol liquid diet to construct alcoholic liver injury model in mice.Cy-3-G was given to mice by daily gavage.Histopathological changes in the liver were observed,biochemical parameters such as levels of total cholesterol(TC)in the liver,levels of serum aspartate aminotransferase(AST),alanine aminotransferase(ALT),and serum endotoxin(LPS)were measured,levels of the pro-inflammatory cytokines interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and macrophage chemotactic protein-1(MCP-1)in the liver were detected,and the death of mice was recorded.Result Short-term ethanol feeding caused significant hepatic steatosis and increased liver TC,serum ALT and AST levels in mice,while Cy-3-G intervention alleviated ethanol-induced hepatic steatosis and reduced liver TC,serum ALT and AST levels in mice.Compared to the ethanol group,Cy-3-G supplementation reduced serum LPS levels,and ameliorated ethanol-induced hepatic inflammation response and reduced IL-1β,TNF-αand MCP-1 levels.Long-term excessive ethanol intake increased mortality of mice in the EtOH group,while mortality in the EtOH+Cy-3-G group was significantly reduced compared to the EtOH group.Conclusion The bioactive substances Cy-3-G plays a protective role on liver injury induced by short-term excessive ethanol consumption in mice,which may be achieved by inhibiting the release of LPS and thus inhibiting the inflammatory response of the liver.In addition,Cy-3-G can significantly prevent the death of mice caused by long-term excessive ethanol consumption and protect health,suggesting that Cy-3-G are expected to be an effective measure against alcoholic liver disease.

关 键 词：矢车菊素-3-O-葡萄糖苷 花色苷 酒精性肝病 内毒素 NIAAA模型 

分 类 号：TS201.4[轻工技术与工程—食品科学] R575[轻工技术与工程—食品科学与工程]