痰瘀同治方的处方优化及验证研究  

Research on Prescription Optimization and Verification of Tanyu Tongzhi Recipe

作　　者：杨湘蕃 张华敏[2] 唐丹丽[3] 李想[4] 李盈盈 吴浩南 刘思鸿[4] YANG Xiangfan;ZHANG Huamin;TANG Danli;LI Xiang;LI Yingying;WU Haonan;LIU Sihong(Yunnan University of Chinese Medicine,Kunming 650500,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beiijng 100700,China;Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Information on Traditional Chinese Medicine,China Academy of Chinese Medical Sciences,Beiijng 100700)

机构地区：[1]云南中医药大学,昆明650500 [2]中国中医科学院中药研究所,北京100700 [3]中国中医科学院医学实验中心,北京100700 [4]中国中医科学院中医药信息研究所,北京100700

出　　处：《中国中医基础医学杂志》2022年第7期1067-1071,1149,共6页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金面上项目(81873199)-基于群体协同计算的痰瘀同治方抗心肌缺血再灌注损伤的处方优化研究;中国中医科学院科技创新工程重大公关项目(CI2021A00604)-从肠道菌群失衡及其介导的胆固醇代谢及炎症反应途径探讨冠心病痰瘀互结病机科学内涵。

摘　　要：目的:对痰瘀同治方进行处方优化研究,并通过动物实验对优化后的处方进行验证。方法:利用课题组前期提出的“以群体协同计算为核心的网络模块划分方法”对痰瘀同治方抗心肌缺血再灌注损伤(myocardial ischemia reperfusion injury,MI/RI)的药理网络进行模块识别,采用加权接近中心性筛选核心节点及核心成分群,最终拟定出优化方1(药物1~6)和优化方2(药物1~5,9,11),随后开展动物实验。将80只SD雄性大鼠随机分为假手术组、MI/RI模型组、痰瘀同治方组、优化方1组及优化方2组,药物干预3周后检测大鼠血清天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、谷丙转氨酶(alanine aminotransferase,ALT)、乳酸脱氢酶(lactate dehydrogenase,LDH)、肌酸激酶(creatine kinase,CK)、肌酸激酶同工酶(creatine kinase-MB,CK-MB)含量;Elisa法测定心肌肌钙蛋白I(cardiac troponin I,cTnI)、白细胞介素(interleukin,IL)-1β和IL-6、肿瘤坏死因子(tumor necrosis factor,TNF)-α含量,比色法检测心肌组织丙二醛(malondialdehyde,MDA)的含量,2,3,5-氯化三苯四唑(2,3,5-triphenyl tetrazolium chlo-ride,TTC)染色法检测心肌梗死面积。结果:与假手术组比较,MI/RI模型组大鼠血清LDH、CK、CK-MB、AST、ALT、MDA、IL-1β、IL-6、TNF-α、cTnI含量和心肌梗死面积显著升高(P<0.01,P<0.05);与MI/RI模型组比较,基础方组和优化方1组各指标值均显著下降(P<0.05,P<0.01),优化方2组的IL-6、TNF-α含量、心肌梗死面积显著下降(P<0.05,P<0.01)。结论:采用“以群体协同为核心的网络模块划分方法”对痰瘀同治方进行优化,得到优化方1和优化方2,动物实验表明优化方1与痰瘀同治方原方疗效相当,证实该处方优化方法切实可行。Objective:The recipe optimization study of Tanyu Tongzhi recipe was carried out,and the optimized recipe was verified by animal experiments.Methods:Using the"network module division method with synergy among multiple components as the core"method to identify the modules of the pharmacological network of Tanyu Tongzhi recipe against myocardial ischemia and reperfusion.The core nodes and core component groups were screened by weighted closeness and finally optimized formula 1(drugs 1~6)and optimized formula 2(drugs 1~5,9,11)were formulated.Then animal experiment was carried out,80 male SD rats were randomly divided into sham operation group,myocardial ischemia reperfusion injury(MI/RI)model group,Tanyu Tongzhi recipe group,optimized recipe 1 group and optimized recipe 2 group.Three weeks after the drug intervention,rat serum AST,ALT,LDH,CK,CK-MB content were detected.Elisa to determine cardiac troponin I(cTnI),IL-1β,IL-6,TNF-αcontents.Colorimetric method to detect MDA content,TTC staining method to detect myocardial infarction area.Results:Compared with the sham operation group,the serum LDH,CK,CK-MB,AST,ALT,MDA,IL-1β,IL-6,TNF-α,cTnI contents and the area of myocardial infarction in the MI/RI model group increased significantly(P<0.05,P<0.01).Compared with the MI/RI model group,all the indexes of the Tanyu Tongzhi recipe group and the optimization recipe 1 group were significantly decreased(P<0.05,P<0.01).The IL-6,TNF-αcontents and the area of myocardial infarction in the optimized 2 group decreased significantly(P<0.05,P<0.01).Conclusion:The study used the"network module division method with synergy among multiple components as the core"method to optimize TanYu Tongzhi recipe,and obtained optimized recipe 1 and optimized recipe 2.Experiments show that the optimized recipe 1 is equivalent to the original Tanyu Tongzhi recipe,which proves that the recipe optimization method is feasible.

关 键 词：痰瘀同治方 处方优化 群体协同计算 

分 类 号：R222.19[医药卫生—中医基础理论]