一株好氧反硝化细菌的筛选鉴定及其脱氮特性  

Screening and identification of an aerobic denitrifying bacteria andits nitrogen removal characteristics

作　　者：朱红旭 杨本芹 高媛 翟红阳 赵艳卿 ZHU Hongxu;YANG Benqing;GAO Yuan;ZHAI Hongyang;ZHAO Yanqing(Faculty of Environmental Science and Engineering,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学环境科学与工程学院,昆明650500

出　　处：《生物学杂志》2022年第4期38-43,共6页Journal of Biology

基　　金：云南省农业环境污染控制与生态修复工程实验室开放基金项目;云南省农田无公害生产创新团队项目(编号:2017HC015)。

摘　　要：从滇池湿地植物根系中分离得到一株好氧反硝化细菌(Aeromonas),编号为RC-15。通过聚合酶链式反应(Polymerase chain reaction,PCR)对该菌株的关键反硝化酶基因进行鉴定,且对该菌株在不同环境因子(碳源、温度、初始pH值、碳氮比)条件下的反硝化能力进行探究。结果显示,该菌株成功扩增出反硝化酶基因nap A和nir K,说明该菌株在有氧分子存在的条件下具有好氧反硝化能力。通过单因素试验得到在碳源为乙酸钠、温度为30℃、初始pH值为8.0、碳氮比为7的培养条件下该菌株反硝化能力最强,24 h时硝氮的去除率达到95.57%,对应的硝氮去除速率达到14.62 mg/(L·h)。研究筛选的菌株RC-15为后续投加高效反硝化菌株强化实际废水脱氮提供了新型菌源。In order to establish the foundation of new biological nitrogen removal process,a strain of Aeromonas was isolated from the roots of wetland plants in Dianchi Lake and was named as RC-15.The key denitrifying enzyme genes of the strain were studied by polymerase chain reaction(PCR),and the denitrification ability of the strain under different environmental conditions(carbon source,temperature,rotation speed,initial pH and C/N ratio)was studied.The results showed that the denitrifying enzyme genes,nap A and nir K were successfully amplified,showing the strain had aerobic denitrification ability in the presence of oxygen molecules.The single factor experiment showed that the strongest denitrification ability was observed with the carbon source of sodium acetate,temperature of 30℃,initial pH of 8.0,and the C/N ratio of 7.Under those conditions,the removal ratio of nitrate reached 95.57%and its corresponded removal rate was 14.62 mg/(L·h)during the initial 24 h.In addition,the strain could remove nitrogen with low temperature,weak acid environment and low C/N.The identified strain RC-15 in this study could provide a new bacterial source to enhance the denitrification of actual wastewater.

关 键 词：好氧反硝化 气单胞菌 功能基因 环境因子 脱氮特性 

分 类 号：X172[环境科学与工程—环境科学] X703