Revisiting the role of delta-1-pyrroline-5-carboxylate synthetase in drought-tolerant crop breeding  

作　　者：Xuanjun Feng Yue Hu Weixiao Zhang Rongqian Xie Huarui Guan Hao Xiong Li Jia Xuemei Zhang Hanmei Zhou Dan Zheng Ying Wen Qingjun Wang Fengkai Wu Jie Xu Yanli Lu 

机构地区：[1]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Wenjiang 611130,Sichuan,China [2]Maize Research Institute of Sichuan Agricultural University,Wenjiang 611130,Sichuan,China

出　　处：《The Crop Journal》2022年第4期1213-1218,共6页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(U21A20209 and 31871640);the key Research Program of the Department of Science and Technology of Sichuan province,China(2021YFYZ0017,2020YJ0350,and 2021YFH0053);the Foundation of International Cooperation in Science and Technology of Chengdu,China(2020-GH02-00027-HZ)。

摘　　要：The delta-1-pyrroline-5-carboxylate synthetase(P5CS)gene exercises a protective function in stressed plants.However,the relationship between proline accumulation caused by P5CS and abiotic stress tolerance in plants is not always clear,as P5CS overexpression has been reported to repress plant growth under normal conditions in several reports.We re-evaluated the role of P5CS in drought-tolerant rice breeding by expressing the AtP5CS1 and feedback-inhibition-removed AtP5CS1(AtP5CS1^(F128A))genes under the regulation of an ABA-inducible promoter to avoid the potential side effects of P5CS overexpression under normal conditions.ABA-inducible AtP5CS1 and AtP5CS1^(F128A) increased seedling growth in a nutrient solution(under osmotic stress)and grain yield in pot plants.However,the evidently deleterious effects of AtP5CS1 on grain quality,tiller number,and grain yield in the field indicated the unsuitability of P5CS for drought-tolerance breeding.

关 键 词：P5CS PROLINE DROUGHT Tiller number CHALKINESS 

分 类 号：S332.4[农业科学—作物遗传育种]