湖南省部分地区养猪场PEDV的检测及S1基因序列变异分析  

Detection of Porcine epidemic diarrhea virus in pig farms in part areas of Hunan Province and S1 gene sequence variation analysis

作　　者：彭娜娜 王文 范方程 宁慧敏 邓瑞德 董伟[1] PENG Nana;WANG Wen;FAN Fangcheng;NING Huimin;DENG Ruide;DONG Wei(College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学动物医学院,长沙410128

出　　处：《黑龙江畜牧兽医》2022年第14期93-96,99,共5页Heilongjiang Animal Science And veterinary Medicine

摘　　要：为了解2020—2021年湖南省部分地区猪流行性腹泻病毒(PEDV)遗传变异情况,试验从10个暴发仔猪腹泻的猪场采集40份样品进行PEDV核酸检测,统计阳性率,并对部分阳性样品的S1基因进行扩增、测序,并进行序列同源性分析和遗传进化分析。结果表明:场阳性率为60.0%,40份组织样品中有21份为PEDV阳性,阳性率为52.5%;6个阳性猪场随机挑选的6份样品PEDV毒株S1基因扩增得到大小均为1 700 bp左右的目的条带,其核苷酸和对应推导的氨基酸序列相似性分别为97.2%~99.8%和95.4%~99.5%,与国内外流行的PEDV变异株(G2型)对应核苷酸序列相似性分别为96.8%~99.2%和95.0%~98.7%;与PEDV疫苗株(CV777株,G1型)对应核苷酸序列相似性分别为91.2%~92.3%和88.9%~90.8%。进一步遗传进化分析结果发现,获得的6个PEDV毒株均属于G2亚群,与我国流行的PEDV变异株所属分支相隔很近。说明PEDV是导致湖南省部分地区仔猪腹泻的常见病原,且均为PEDV变异株。In order to understand the genetic variation of Porcine epidemic diarrhea virus(PEDV) in part areas of Hunan Province from 2020 to 2021, 40 samples were collected from 10 pig farms with outbreaks of piglet diarrhea for PEDV nucleic acid detection;the positive rate was counted, and the S1 gene of some positive samples was amplified and sequenced;sequence homology analysis and genetic evolution analysis were carried out. The results showed that the farm positive rate was 60.0%;21 of the 40 tissue samples were PEDV positive, and the positive rate was 52.5%. The S1 gene of PEDV strain was amplified from 6 samples which were randomly selected from 6 positive pig farms, and the target bands were all about 1 700 bp in size. The nucleotide and corresponding deduced amino acid sequence similarity were 97.2%-99.8% and 95.4%-99.5%, respectively;the corresponding sequence similarity with the popular PEDV variant(G2 type) at home and abroad was 96.8%-99.2% and 95.0%-98.7%, respectively, and the corresponding sequence similarity with PEDV vaccine strain(CV777 strain, G1 type) was 91.2%-92.3% and 88.9%-90.8%, respectively. Further genetic evolution analysis showed that the obtained 6 PEDV strains belonged to the G2 subgroup, which was very close to the branch to which the PEDV variant strains prevailed in China. The results suggested that PEDV was a common pathogen causing piglet diarrhea in part areas of Hunan Provice, and all of them were PEDV mutants.

关 键 词：猪流行性腹泻病毒 S1基因 遗传变异 系统进化分析 湖南省 

分 类 号：S852.65[农业科学—基础兽医学]